PXD050043-1

PXD050043 is an original dataset announced via ProteomeXchange.

Title	Comprehensive characterization of raw and processed quinoa from conventional and organic farming by label-free shotgun proteomics
Description	Quinoa is widely recognized for its exceptional nutritional properties, particularly its complete protein content. This study, for the first time, investigates the effects of processing methods (boiling and extrusion) and farming conditions (conventional and organic) on the quinoa’s proteomic profile. Following a label-free shotgun proteomics approach, a total of 1,796 proteins were identified and quantified across all quinoa samples. Regarding processing, both boiling and extrusion produced protein extracts with lower total protein content, with the number of identified proteins decreasing from 1,695 in raw quinoa to 957 in processed quinoa. Boiling led to a reduction in protein diversity and expression, while extrusion, which involves high temperatures and pressures, specifically decreased the abundance of high molecular mass proteins. Concerning cultivation practices, organic farming was associated with a broader protein diversity, especially proteins related to translation (28% vs. 5%), while conventional farming showed a higher abundance of catalytic and enzymatic proteins (67% vs. 46%). These findings highlight the distinct proteomic changes induced by different processing methods and farming conditions, offering valuable insights to manage quinoa’s nutritional, bioactive, and functional properties across various production practices.
HostingRepository	PRIDE
AnnounceDate	2025-01-17
AnnouncementXML	Submission_2025-01-17_04:34:18.422.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zoran Minic
SpeciesList	scientific name: Chenopodium quinoa; NCBI TaxID: 63459;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-22 10:39:34	ID requested
⏵ 1	2025-01-17 04:34:18	announced

10.1021/ACS.JAFC.4C08623;

submitter keyword: Raw quinoa, NanoLC-MS/MS, Processed quinoa,Shotgun proteomics, Conventional farming, Organic farming

Laura Pont
contact affiliation	Department of Chemical Engineering and Analytical Chemistry, Institute for Research on Nutrition and Food Safety (INSA·UB), University of Barcelona, 08028 Barcelona, Spain
contact email	laura.pont@ub.edu
lab head
Zoran Minic
contact affiliation	John L. Holmes Mass Spectrometry Facility, University of Ottawa
contact email	zminic@uottawa.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD050043

PRIDE project URI

• PRIDE
  1. PXD050043
     1. Label: PRIDE project
     2. Name: Comprehensive characterization of raw and processed quinoa from conventional and organic farming by label-free shotgun proteomics