PXD050004-1
PXD050004 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of NKG7-proximal proteins by TurboID |
| Description | NKG7 is a lysosomal membrane protein whose expression is restricted to cytotoxic lymphocytes. Although the importance of NKG7 in anti-tumor immunity has been shown, little is known about the molecular function and regulation of NKG7. To elucidate NKG7 molecular function, we performed NKG7-proximity labeling using TurboID system in 293T cells, followed by mass-spectrometry. We identified vacuolar H+ ATPase (v-ATPase; lysosomal proton pump) subunit ATP6AP2 as a potential NKG7-interacting protein, and validated the interaction via exogenous coimmunoprecipitation. We further showed that NKG7 inhibits v-ATPase activity, thereby impacting lysosomal homeostasis and mTORC1 regulation at lysosomes. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-01-29 |
| AnnouncementXML | Submission_2025-01-29_08:24:24.394.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Kenneth L. Johnson |
| SpeciesList | scientific name: Human; |
| ModificationList | Biotin; L-methionine sulfoxide; Carbamidomethyl |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-02-21 07:44:21 | ID requested | |
| ⏵ 1 | 2025-01-29 08:24:24 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: TurboID, proximity labeling, NKG7, cytotoxic lymphocytes, lysosome |
Contact List
| Daniel Billadeau, Ph.D. | |
|---|---|
| contact affiliation | Mayo Clinic, Rochester, MN |
| contact email | Billadeau.Daniel@mayo.edu |
| lab head | |
| Kenneth L. Johnson | |
| contact affiliation | Mayo Clinic |
| contact email | johnson.kenneth@mayo.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094141/ |
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