PXD050003-1

PXD050003 is an original dataset announced via ProteomeXchange.

Title	Inflammatory proteins and neutrophil extracellular traps increase in burn blister fluid after 24 hours post-burn
Description	Burn wound blister fluid is a valuable matrix for understanding the biological pathways associated with burn injury. In this study, 152 blister fluid samples collected from paediatric burn wounds at two different hospitals were analysed using mass spectrometry proteomic techniques. The protein abundance profile at different days post-burn indicated that there were more proteins associated with cellular damage/repair in the first 24 hours, whereas after this point there were more proteins associated with antimicrobial defence and inflammation. The inflammatory proteins persisted at a high level in the blister fluid for more than 7 days. This may indicate that removal of burn blisters prior to two days post-burn is optimal to prevent excessive or prolonged inflammation in the wound environment. Additionally, many proteins associated with the neutrophil extracellular trap (NET) pathway were increased post-burn, further implicating NETs in the post-burn inflammatory response. NET inhibitors may therefore be a potential treatment to reduce post-burn inflammation and coagulation pathology and enhance burn wound healing outcomes.
HostingRepository	PRIDE
AnnounceDate	2024-03-04
AnnouncementXML	Submission_2024-03-03_23:56:10.814.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leila Cuttle
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-21 07:19:58	ID requested
⏵ 1	2024-03-03 23:56:11	announced

10.1016/J.BURNS.2024.02.026;

submitter keyword: proteomics, child, wound fluid, Paediatric,SWATH, LCMS, burn

Leila Cuttle
contact affiliation	Queensland University of Technology (QUT), Faculty of Health, School of Biomedical Sciences, Centre for Children’s Health Research
contact email	leila.cuttle@qut.edu.au
lab head
Leila Cuttle
contact affiliation	Queensland University of Technology
contact email	leila.cuttle@qut.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD050003

PRIDE project URI

• PRIDE
  1. PXD050003
     1. Label: PRIDE project
     2. Name: Inflammatory proteins and neutrophil extracellular traps increase in burn blister fluid after 24 hours post-burn