PXD049970 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Carrier protein-enabled host cell proteome analysis (CP-HCP) with integrated isobaric tag interference filtering |
| Description | We used TMT labelling to set up different experiment channels containing mixtures of the host cell carrier proteome (E coli) and mock biotherapeutic (BSA), to compare with interference detection channel to model and filter interference, a phenomenon where host cell proteins (HCPs) co-elute with high-background biotherapeutic during LC-MS monitoring of QC during biomanufacture of biopharmaceuticals. Our work overcomes limitations of ELISA and pre-tryptic digest procedures to produce a reproducible method using TMT labels to identify and quantify a range of HCPs in one experiment. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-12 |
| AnnouncementXML | Submission_2026-01-11_16:08:17.603.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Divyanshi Karmani |
| SpeciesList | scientific name: Escherichia coli; NCBI TaxID: NEWT:562; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-02-20 11:36:41 | ID requested | |
| ⏵ 1 | 2026-01-11 16:08:18 | announced | |
Publication List
| Karmani D, Seifihesar N, Sultonova M, Blackmore B, Paulo JA, Harty M, Murphy JP, Carrier-Guided Proteome Analysis in a High Protein Background: An Improved Approach to Host Cell Protein Identification. J Proteome Res, 23(11):5193-5202(2024) [pubmed] |
| 10.1021/acs.jproteome.4c00158; |
Keyword List
| submitter keyword: BOOST,host cell proteins, multi-plexed proteomics, TMT, carrier proteome, LC-MS, co-isolation, interference, co-fragmentation, DDA |
Contact List
| John Patrick Murphy |
|---|
| contact affiliation | Murphy Lab, Department of Biology, Duffy Science Center, University of Prince Edward Island, PE, Canada C1A4P3. |
| contact email | jpmurphy@upei.ca |
| lab head | |
| Divyanshi Karmani |
|---|
| contact affiliation | Graduate Student, Murphy Lab, Duffy Science Center, Department of Biology, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE, C1A 4P3 |
| contact email | dkarmani@upei.ca |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD049970 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD049970
- Label: PRIDE project
- Name: Carrier protein-enabled host cell proteome analysis (CP-HCP) with integrated isobaric tag interference filtering
