PXD049968-1
PXD049968 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Thylakoid Protein FPB1 Synergistically Cooperates with PAM68 to Promote CP47 Biogenesis and Photosystem II Assembly |
| Description | LC-MS/MS analysis was used for protein identification of immunoprecipitation. 20 uL eluted samples of immunoprecipitation were separated by 12.5% SDS-PAGE and each gel lane was cut in consecutive gel slices followed by destaining twice using NH4HCO3/ACN. Proteins were digested in-gel with DTT, IAA and trypsin. Digested peptides in the gel slices were then desalted using StageTips with C18 Cartridge (Sigma). Peptides extracts for each gel slice were analyzed by High Performance Liquid Chromatography (HPLC) using EASY column (Fisher Scientific, USA) coupled with a Q-Exactive mass spectrometer (Thermo Finnigan). Resulting MS/MS spectra were searched against the predicted Arabidopsis proteome (35386 entries, 20191018) with maxquant software (version 2.3). |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-02-20 |
| AnnouncementXML | Submission_2024-02-20_11:06:38.229.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Lin Zhang |
| SpeciesList | scientific name: Arabidopsis thaliana; common name: thale cress; NCBI TaxID: 3702; |
| ModificationList | unknown modification |
| Instrument | instrument model |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-02-20 10:42:19 | ID requested | |
| ⏵ 1 | 2024-02-20 11:06:38 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: FPB1, Shortgun, coIP |
Contact List
| Lin Zhang | |
|---|---|
| contact affiliation | Shanghai Normal University |
| contact email | zhanglin2017@shnu.edu.cn |
| lab head | |
| Lin Zhang | |
| contact affiliation | Shanghai Normal University |
| contact email | zhanglin2017@shnu.edu.cn |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094131/ |
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