PXD049478-1

PXD049478 is an original dataset announced via ProteomeXchange.

Title	Protein aggregation is a consequence of the dormancy-inducing membrane toxin TisB in Escherichia coli
Description	Protein aggregates occur in all living cells due to misfolding of proteins. In bacteria, protein aggregation is associated with cellular inactivity, which is related to dormancy and tolerance to stressful conditions, including the exposure to antibiotics. In Escherichia coli, the membrane toxin TisB is an important factor for dormancy and antibiotic tolerance upon DNA damage mediated by the fluoroquinolone antibiotic ciprofloxacin. Here, we show that TisB provokes protein aggregation in response to ciprofloxacin. The stress response and TisB-dependent protein aggregates are analyzed by LC-MS.
HostingRepository	PRIDE
AnnounceDate	2024-10-04
AnnouncementXML	Submission_2024-10-04_05:15:01.394.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Tholey
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-19 04:00:55	ID requested
⏵ 1	2024-10-04 05:15:01	announced
2	2024-10-22 07:00:43	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: toxin-antitoxin system, antibiotics, dormancy, protein aggregation, type I toxin

Andreas Tholey
contact affiliation	Institut für Experimentelle Medizin (IEM) Arbeitsgruppe Systematische Proteomforschung Christian-Albrechts-Universität zu Kiel Niemannsweg 11 24105 Kiel, Germany
contact email	a.tholey@iem.uni-kiel.de
lab head
Andreas Tholey
contact affiliation	Systematic Proteome Research & Bioanalytics, University of Kiel
contact email	a.tholey@iem.uni-kiel.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049478
     1. Label: PRIDE project
     2. Name: Protein aggregation is a consequence of the dormancy-inducing membrane toxin TisB in Escherichia coli