PXD049473-1

PXD049473 is an original dataset announced via ProteomeXchange.

Title	An outer membrane porin-lipoprotein complex modulates elongasome movement to establish cell curvature in Rhodospirillum rubrum
Description	Curved cell shapes are widespread among bacteria and important for cellular motility, virulence and fit-ness. However, the underlying morphogenetic mechanisms are still incompletely understood. Here, we identify an outer-membrane protein complex that promotes cell curvature in the pho¬tosynthetic species Rhodo¬spirillum rubrum. We show that the R. rubrum porins Por39 and Por41 form helical ribbon-like struc-tures at the outer curve of the cell that recruit the peptido¬glycan-binding lipo¬pro¬tein PapS, with PapS in-activation, porin de¬localization or disruption of the porin-PapS interface resulting in cell straight¬ening. We further demonstrate that porin-PapS assemblies act as molecular cages that entrap the cell elon¬gation ma-chine¬ry, thus biasing cell growth towards the outer curve. These findings reveal a mechanistically dis¬tinct mor¬pho¬genetic module medi¬ating bacterial cell shape. Moreover, they uncover an unprecedented role of outer-mem¬brane pro¬tein pat¬terning in the spatial control of intracellular processes, adding an important facet to the reper¬toire of regu¬la¬tory mechanisms in bac¬terial cell biology.
HostingRepository	PRIDE
AnnounceDate	2024-07-17
AnnouncementXML	Submission_2024-07-17_04:40:53.903.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Timo Glatter
SpeciesList	scientific name: Rhodospirillum rubrum; NCBI TaxID: 1085;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-19 02:36:15	ID requested
⏵ 1	2024-07-17 04:40:54	announced
2	2024-10-22 06:50:39	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: peptidoglycan,Bacteria, morphogenesis, cell wall, cell shape

Timo Glatter
contact affiliation	Max Planck Institute for Terrestrial Microbiology Karl-von-Frisch Str. 10 35043 Marburg Germany
contact email	timo.glatter@mpi-marburg.mpg.de
lab head
Timo Glatter
contact affiliation	Max Planck Institute for Terrestrial Microbiology Marburg
contact email	timo.glatter@mpi-marburg.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD049473

PRIDE project URI

• PRIDE
  1. PXD049473
     1. Label: PRIDE project
     2. Name: An outer membrane porin-lipoprotein complex modulates elongasome movement to establish cell curvature in Rhodospirillum rubrum