PXD049454-1
PXD049454 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The natural product co-evolved pyrroloquinoline quinone operon enhances their production when heterologously expressed in a variety of Streptomycetes |
| Description | Streptomyces has the largest repertoire of natural product biosynthetic gene clusters (BGCs), yet developing a universal engineering strategy for each Streptomyces species is challenging. Given that some Streptomyces species have larger BGC repertoires than others, we hypothesized that a set of genes co-evolved with BGCs to support biosynthetic proficiency must exist in those strains, and that their identification may provide universal strategies to improve the productivity of other strains. We show here that genes co-evolved with natural product BGCs in Streptomyces can be identified by phylogenomics analysis. Among the 597 genes that co-evolved with polyketide BGCs, 11 genes in the “coenzyme” category have been examined, including a gene cluster encoding for the co-factor pyrroloquinoline quinone (PQQ). When the pqq gene cluster was engineered into 11 Streptomyces strains, it enhanced production of 16,385 metabolites, including 36 known natural products with up to 40-fold improvement and several activated silent gene clusters. This study provides a new engineering strategy for improving polyketide production and discovering new biosynthetic gene clusters. |
| HostingRepository | iProX |
| AnnounceDate | 2024-02-12 |
| AnnouncementXML | Submission_2024-02-17_23:15:43.213.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xiaozhou Luo |
| SpeciesList | scientific name: Streptomyces coelicolor A3(2); NCBI TaxID: 100226; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-02-17 23:15:18 | ID requested | |
| ⏵ 1 | 2024-02-17 23:15:43 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: PQQ, natural products, streptomyces |
Contact List
| Xiaozhou Luo | |
|---|---|
| contact affiliation | Shenzhen Institute of Advanced Technology |
| contact email | xz.luo@siat.ac.cn |
| lab head | |
| Xiaozhou Luo | |
| contact affiliation | Shenzhen Institute of Advanced Technology |
| contact email | xr.wang@siat.ac.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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