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PXD049454 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe natural product co-evolved pyrroloquinoline quinone operon enhances their production when heterologously expressed in a variety of Streptomycetes
DescriptionStreptomyces has the largest repertoire of natural product biosynthetic gene clusters (BGCs), yet developing a universal engineering strategy for each Streptomyces species is challenging. Given that some Streptomyces species have larger BGC repertoires than others, we hypothesized that a set of genes co-evolved with BGCs to support biosynthetic proficiency must exist in those strains, and that their identification may provide universal strategies to improve the productivity of other strains. We show here that genes co-evolved with natural product BGCs in Streptomyces can be identified by phylogenomics analysis. Among the 597 genes that co-evolved with polyketide BGCs, 11 genes in the “coenzyme” category have been examined, including a gene cluster encoding for the co-factor pyrroloquinoline quinone (PQQ). When the pqq gene cluster was engineered into 11 Streptomyces strains, it enhanced production of 16,385 metabolites, including 36 known natural products with up to 40-fold improvement and several activated silent gene clusters. This study provides a new engineering strategy for improving polyketide production and discovering new biosynthetic gene clusters.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXiaozhou Luo
SpeciesList scientific name: Streptomyces coelicolor A3(2); NCBI TaxID: 100226;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-02-17 23:15:18ID requested
12024-02-17 23:15:43announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: PQQ, natural products, streptomyces
Contact List
Xiaozhou Luo
contact affiliationShenzhen Institute of Advanced Technology
contact emailxz.luo@siat.ac.cn
lab head
Xiaozhou Luo
contact affiliationShenzhen Institute of Advanced Technology
contact emailxr.wang@siat.ac.cn
dataset submitter
Full Dataset Link List
iProX dataset URI