PXD049415-1

PXD049415 is an original dataset announced via ProteomeXchange.

Title	Characterization of a lipid-based jumbo phage compartment as a hub for early phage infection
Description	Viral genomes are most vulnerable to cellular defenses at the start of the infection. A family of jumbo phages related to phage ΦKZ, which infects Pseudomonas aeruginosa, assembles a protein-based phage nucleus to protect replicating phage DNA, but how it is protected prior to phage nucleus assembly is unclear. We find that host proteins related to membrane and lipid biology interact with injected phage protein, clustering in an early phage infection (EPI) vesicle. The injected virion RNA polymerase (vRNAP) executes early gene expression until phage genome separation from the vRNAP and the EPI vesicle, moving into the nascent proteinaceous phage nucleus. Enzymes involved in DNA replication and CRISPR/restriction immune nucleases are excluded by the EPI vesicle. We propose that the EPI vesicle is rapidly constructed with injected phage proteins, phage DNA, host lipids, and host membrane proteins to enable genome protection, early transcription, localized translation, and to ensure faithful genome transfer to the proteinaceous nucleus.
HostingRepository	PRIDE
AnnounceDate	2024-11-26
AnnouncementXML	Submission_2024-11-25_20:59:55.357.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Danielle Swaney
SpeciesList	scientific name: Pseudomonas phage phiKZ; NCBI TaxID: 2905945; scientific name: Pseudomonas aeruginosa PAO1-VE2; NCBI TaxID: 1367493;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-15 20:59:40	ID requested
⏵ 1	2024-11-25 20:59:56	announced

10.1016/j.chom.2024.05.016;

Mozumdar D, Fossati A, Stevenson E, Guan J, Nieweglowska E, Rao S, Agard D, Swaney DL, Bondy-Denomy J, Characterization of a lipid-based jumbo phage compartment as a hub for early phage infection. Cell Host Microbe, 32(7):1050-1058.e7(2024) [pubmed]

submitter keyword: APMS, Pseudomonas aeruginosa,Phage

Danielle Swaney
contact affiliation	UCSF
contact email	daniswan@gmail.com
lab head
Danielle Swaney
contact affiliation	UCSF
contact email	daniswan@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD049415

PRIDE project URI

• PRIDE
  1. PXD049415
     1. Label: PRIDE project
     2. Name: Characterization of a lipid-based jumbo phage compartment as a hub for early phage infection