PXD049377 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Differentiating children with sepsis with and without acute respiratory distress syndrome using proteomics |
Description | Both sepsis and acute respiratory distress syndrome (ARDS) rely on imprecise clinical definitions leading to heterogeneity, which has contributed to negative trials. Because circulating protein/DNA complexes have been implicated in sepsis and ARDS, we aimed to develop a proteomic signature of DNA-bound proteins to discriminate between children with sepsis with and without ARDS. We performed a prospective case-control study in 12 children with sepsis with ARDS matched to 12 children with sepsis without ARDS on age, severity of illness score, and source of infection. We performed co-immunoprecipitation and downstream proteomics in plasma collected ≤ 24 h of intensive care unit admission. Expression profiles were generated, and a random forest classifier was used on differentially expressed proteins to develop a signature which discriminated ARDS. The classifier was tested in six independent blinded samples. Neutrophil and nucleosome proteins were over-represented in ARDS, including two S100A proteins, superoxide dismutase (SOD), and three histones. Random forest produced a 10-protein signature that accurately discriminated between children with sepsis with and without ARDS. This classifier perfectly assigned six independent blinded samples as having ARDS or not. We validated higher expression of the most informative discriminating protein, galectin-3-binding protein, in children with ARDS. Our methodology has applicability to isolation of DNA-bound proteins from plasma. Our results support the premise of a molecular definition of ARDS, and give preliminary insight into why some children with sepsis, but not others, develop ARDS. |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-14 |
AnnouncementXML | Submission_2024-02-14_08:48:33.713.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hossein Fazelinia |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-02-14 08:14:55 | ID requested | |
⏵ 1 | 2024-02-14 08:48:34 | announced | |
Publication List
Yehya N, Fazelinia H, Taylor DM, Lawrence GG, Spruce LA, Thompson JM, Margulies SS, Seeholzer SH, Worthen GS, Differentiating children with sepsis with and without acute respiratory distress syndrome using proteomics. Am J Physiol Lung Cell Mol Physiol, 322(3):L365-L372(2022) [pubmed] |
10.1152/ajplung.00164.2021; |
Keyword List
submitter keyword: Nucleosome, DNA-bound proteins, acute respiratory distress syndrome,Sepsis, Neutrophil |
Contact List
Nadir Yehya, MD |
contact affiliation | Department of Anesthesiology and Critical Care Medicine Children’s Hospital of Philadelphia University of Pennsylvania Philadelphia, Pennsylvania |
contact email | yehyan@email.chop.edu |
lab head | |
Hossein Fazelinia |
contact affiliation | Children's Hospital of Philadelphia |
contact email | fazeliniah@email.chop.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD049377 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD049377
- Label: PRIDE project
- Name: Differentiating children with sepsis with and without acute respiratory distress syndrome using proteomics