PXD049372-1

PXD049372 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Identification of the Drosophila centriolar satellite proximity interactome by direct and indirect biotin proximity labeling
Description	In this set of experiments we identified the main scaffolding component of centriolar satellites, PCM1 or Cmb in Drosophila as a proximity interactor of the centriolar structural protein Sas4 by BioID in S2 cells and subsequently explored the Cmb proximity interactome by direct TurboID in S2 cells and indirect, GFP nanobody-targeted, TurboID in Drosophila testes.
HostingRepository	PRIDE
AnnounceDate	2025-03-31
AnnouncementXML	Submission_2025-03-31_00:30:59.775.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Hartl
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Exploris 480; Q Exactive HF-X

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-14 06:27:31	ID requested
⏵ 1	2025-03-31 00:31:00	announced

Publication List

10.1083/JCB.202408042;

10.1083/JCB.202408042;

Keyword List

submitter keyword: D. melanogaster
Centrosome
Centriolar satellites
Interaction mapping
BioID
TurboID

submitter keyword: D. melanogaster

Centrosome

Centriolar satellites

Interaction mapping

BioID

TurboID

Contact List

Alexander Dammermann
contact affiliation	Max Perutz Labs, University of Vienna, Vienna Biocenter (VBC), Dr Bohr-Gasse 9, A-1030 Vienna, Austria
contact email	alex.dammermann@univie.ac.at
lab head
Markus Hartl
contact affiliation	Max Perutz Labs, Mass Spectrometry Facility, Vienna Biocenter Campus (VBC), Dr. Bohr-Gasse 9, Vienna, Austria
contact email	proteomics@maxperutzlabs.ac.at
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD049372
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD049372

PRIDE project URI

Repository Record List

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