PXD049371 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | MS-based proteomic characterization of assembled microtubules |
Description | Microtubules are dynamic polymers that interconvert between phases of growth and shrinkage, yet they somehow provide lasting structural stability to cells. Growth involves hydrolysis of GTP-tubulin to GDP-tubulin, which releases energy that is stored within the microtubule lattice and destabilizes it; a GTP cap at microtubule ends is thought to prevent GDP subunits from rapidly dissociating and causing catastrophe. We show that GDP-tubulin, usually considered as an inactive tubulin species, can itself assemble into microtubules preferentially at the minus end, and promote persistent growth. We characterized by mass spectrometry-based proteomics the protein content of tubulin purified from bovine brain (Total) and of microtubules grown from stable GMPCPP seeds in the presence of either GDP-tubulin or GTP-tubulin. |
HostingRepository | PRIDE |
AnnounceDate | 2024-04-30 |
AnnouncementXML | Submission_2024-04-30_09:18:00.997.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD049371 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Yohann Couté |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-02-14 06:20:03 | ID requested | |
⏵ 1 | 2024-04-30 09:18:01 | announced | |
Publication List
Keyword List
submitter keyword: Microtubules, tubulin, purification, in-vitro assembly, nanoLC-MS/MS |
Contact List
Yohann Couté |
contact affiliation | EDyP |
contact email | yohann.coute@cea.fr |
lab head | |
Yohann Couté |
contact affiliation | EDyP |
contact email | yohann.coute@cea.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD049371
- Label: PRIDE project
- Name: MS-based proteomic characterization of assembled microtubules