PXD049365-1

PXD049365 is an original dataset announced via ProteomeXchange.

Title	SRF is a new player in 3D genome organization and stem cell pluripotency
Description	Serum response factor (SRF) is a transcription factor essential for cell proliferation, differentiation, and migration, and is required for primitive streak and mesoderm formation in the embryo. The canonical roles of SRF are mediated by a diverse set of context-dependent cofactors. Here we show that SRF physically interacts with CTCF and cohesin subunits at TAD boundaries and loop anchors. SRF reinforces the insulation of TADs and promotes the formation of long-range chromatin loops. In ES cells, SRF associates with Oct4, Sox2, and Nanog and contributes to the formation of 3D pluripotency hubs. Our findings reveal new roles of SRF in higher-order chromatin organization.
HostingRepository	PRIDE
AnnounceDate	2024-10-15
AnnouncementXML	Submission_2024-10-15_01:50:06.522.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Meierhofer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-14 04:35:51	ID requested
⏵ 1	2024-10-15 01:50:07	announced
2	2024-10-22 07:01:46	announced	2024-10-22: Updated project metadata.

10.1016/J.CELREP.2024.114846;

submitter keyword: Serum response factor (SRF)

higher-order chromatin organization

Bernhard Herrmann
contact affiliation	Max-Planck-Institut for Molecular Genetics Department of Developmental Genetics Ihnestraße 63-73 14195 Berlin, Germany
contact email	herrmann@molgen.mpg.de
lab head
David Meierhofer
contact affiliation	Mass Spectrometry Facility MPIMG
contact email	meierhof@molgen.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD049365

PRIDE project URI

• PRIDE
  1. PXD049365
     1. Label: PRIDE project
     2. Name: SRF is a new player in 3D genome organization and stem cell pluripotency