PXD049352-1

PXD049352 is an original dataset announced via ProteomeXchange.

Title	Photoreceptor-induced LHL4 protects photosystem II in Chlamydomonas reinhardtii
Description	Photosynthesis, the fundamental process using light energy to convert CO2 to organic matter, is vital for life on Earth. It relies on capturing light through light-harvesting complexes in photosystems I and II and converting light energy into chemical energy. Composition and organization of photosystem core complexes are well conserved across evolution, and are highly susceptible to photodamage. Consequently, a large diversity of photoprotective mechanisms have evolved in photoautotrophs, finely tuned for the specific light conditions. Light Harvesting Complex protein family (LHC and LHC-like families) have acquired a dual function during evolution. Members of the LHC antenna complexes of photosystems capture light energy whereas others dissipate excess energy that cannot be harnessed for photosynthesis. This process mainly occurs through non photochemical quenching (NPQ). In this work, we focus on the LHL4 protein, which is induced by UV-B and blue light photoreceptor signaling pathways in the model green microalgae Chlamydomonas reinhardtii. Our work demonstrates that alongside established NPQ effectors, LHL4 plays a key role in photoprotection, preventing singlet oxygen accumulation in photosystem II and thereby promoting cell survival upon light stress. LHL4 protective function is distinct from that of NPQ-related proteins, since it directly binds to the transient monomeric form of the core photosystem II complex, safeguarding its integrity. Our LHL4 characterization expands our understanding of the interplay between light harvesting and photoprotection mechanisms upon light stress in photosynthetic microalgae.
HostingRepository	PRIDE
AnnounceDate	2025-01-28
AnnouncementXML	Submission_2025-01-28_00:52:27.597.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pascal Albanese
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-13 06:43:08	ID requested
⏵ 1	2025-01-28 00:52:27	announced

Dataset with its publication pending

submitter keyword: in-gel crosslikning, photosystem II, DMTMM, light-harvesting complexes, XL-MS, thylakoids

Pascal Albanese
contact affiliation	Light, Photosynthesis & Metabolism (LPM) & Exploring the Dynamics of Proteomes (EDyP) IRIG, CEA-Grenoble 17 rue des Martyrs, 38000 Grenoble, France
contact email	pascal.albanese@cea.fr
lab head
Pascal Albanese
contact affiliation	Light, Photosynthesis & Metabolism (LPM) & Exploring the Dynamics of Proteomes (EDyP) IRIG, CEA-Grenoble 17 rue des Martyrs, 38000 Grenoble, France
contact email	pascal.albanese@cea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049352
     1. Label: PRIDE project
     2. Name: Photoreceptor-induced LHL4 protects photosystem II in Chlamydomonas reinhardtii