PXD049346-1

PXD049346 is an original dataset announced via ProteomeXchange.

Title	Identification of ID4-interacting proteins in cultured undifferentiated spermatogonia
Description	ID4 belongs to a family of transcriptional regulators involved in stem cell function. ID family proteins act by binding and inhibiting activity of basic-HLH (bHLH) transcription factors, including E-proteins and class II factors. ID function is also mediated through interaction with non-bHLH proteins. Spermatogonial stem cells (SSCs) are found within a population of undifferentiated spermatogonia in the testis and are essential for the sustained production of sperm over the lifespan. It is reported that ID4 is involved in SSC maintenance but the downstream targets of ID4 in SSCs are unclear. To gain insight into the function of ID4 in SSCs we identified interacting partners in cultures of mouse undifferentiated spermatogonia by immunoprecipitation using a specific ID4 antibody and mass spectrometry analysis.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:09:17.533.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ralf Schittenhelm
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-12 19:37:47	ID requested
⏵ 1	2025-05-06 13:09:18	announced

10.1016/j.stemcr.2024.08.011;

La HM, Chan AL, Hutchinson AM, Su BYM, Rossello FJ, Schittenhelm RB, Hobbs RM, Functionally redundant roles of ID family proteins in spermatogonial stem cells. Stem Cell Reports, 19(10):1379-1388(2024) [pubmed]

submitter keyword: ID family proteins, transcription factors,Spermatogonial stem cells, male fertility

Robin Hobbs
contact affiliation	Centre for Reproductive Health, Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia
contact email	robin.hobbs@monash.edu
lab head
Ralf Schittenhelm
contact affiliation	Monash University
contact email	ralf.schittenhelm@monash.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049346
     1. Label: PRIDE project
     2. Name: Identification of ID4-interacting proteins in cultured undifferentiated spermatogonia