PXD049328 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Trypanosoma brucei whole cell proteome changes upon exposure to the aminomethyl-benzoxaborole AN3057 |
Description | Benzoxaboroles (BoBs) feature a boron-heterocyclic core and are an important recent innovation in the development of drugs against a range of pathogens and other pathologies. A broad spectrum of pharmacology is associated with chemically diverse BoB derivatives and includes multiple modes-of-action and targets. However, a consensus MoA for BoBs targeting evolutionarily diverse protozoan pathogens has emerged with the identification of CPSF3/CPSF73 in the CPSF complex in both apicomplexan and kinetoplastida parasites. We have detected a functional connection between protein sumoylation and the BoB boron-heterocyclic scaffold using comprehensive genetic screens in Trypanosoma brucei. Strikingly, as part of this sumoylation response, members of the CPSF complex are specifically and rapidly destabilised in a SUMO and proteosome-dependent manner. Here we deposit SILAC proteomics data quantifying the effects of BoB exposure on the global protein landscape in T. brucei. |
HostingRepository | PRIDE |
AnnounceDate | 2024-03-25 |
AnnouncementXML | Submission_2024-03-25_03:15:09.072.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Martin Zoltner |
SpeciesList | scientific name: Trypanosoma brucei; NCBI TaxID: 5691; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-02-12 04:10:17 | ID requested | |
⏵ 1 | 2024-03-25 03:15:09 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: benzoxaborole,SUMO, trypanosoma, mode of action, CPSF complex, proteasome |
Contact List
Dr. Martin Zoltner |
contact affiliation | Head of Drug Discovery and Evaluation Charles University in Prague, BIOCEV Průmyslová 595 252 50 Vestec Czech Republic phone: +420 325 873 966 |
contact email | zoltnerm@natur.cuni.cz |
lab head | |
Martin Zoltner |
contact affiliation | Division of Biological Chemistry & Drug Discovery School of Life Sciences University of Dundee Dundee DD1 5EH |
contact email | m.zoltner@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD049328 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD049328
- Label: PRIDE project
- Name: Trypanosoma brucei whole cell proteome changes upon exposure to the aminomethyl-benzoxaborole AN3057