PXD049295-1

PXD049295 is an original dataset announced via ProteomeXchange.

Title	Aptamer-conjugated gold nanoparticles enable selective oligonucleotide delivery into muscle 2 stem cells to promote regeneration of dystrophic muscles
Description	Inefficient targeting of muscle stem cells (MuSCs) represents a major bottleneck of current 27 therapeutic strategies for muscular dystrophies, as it precludes the possibility to promote 28 compensatory regeneration. Here we describe a novel delivery platform, based on gold nanoparticles, 29 that enables the release of therapeutic oligonucleotides into MuSCs. We demonstrate that AuNPs 30 conjugation to an aptamer against  integrin directs either local or systemic delivery of 31 microRNA-206 to MuSCs, thereby promoting muscle regeneration in a mouse model of Duchenne 32 Muscular Dystrophy. We show here that this platform is biocompatible, non-toxic, non33 immunogenic, and adaptable for release of a wide range of therapeutic oligonucleotides into diseased 34 muscles.
HostingRepository	PRIDE
AnnounceDate	2024-11-11
AnnouncementXML	Submission_2024-11-11_02:40:29.846.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD049295
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sergio Ciordia
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-09 04:27:26	ID requested
⏵ 1	2024-11-11 02:40:30	announced

10.6019/PXD049295;

submitter keyword: gold nanoparticles, selex, proteomics, oligonucleotide,Duchenne muscular dystrophy, aptamer

Daniela Palacios
contact affiliation	Università Cattolica del Sacro Cuore, Department of Life Science and Public Health, Rome, Italy
contact email	daniela.palacios@unicatt.it
lab head
Sergio Ciordia
contact affiliation	Spanish National Center for Biotechnology
contact email	sciordia@cnb.csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD049295

PRIDE project URI

• PRIDE
  1. PXD049295
     1. Label: PRIDE project
     2. Name: Aptamer-conjugated gold nanoparticles enable selective oligonucleotide delivery into muscle 2 stem cells to promote regeneration of dystrophic muscles