PXD049246-1

PXD049246 is an original dataset announced via ProteomeXchange.

Title	HelQ-BioID2 experiment to discover novel protein interactants of HelQ
Description	HelQ is a 3’-5’ single stranded DNA (ssDNA) dependent helicase part of the Superfamily 2 helicases (SF2) which are characterised by the presence of two motor domains with a RecA-like fold which couples ATP hydrolysis to DNA translocation. The N-terminal domain of HelQ or N-HelQ (~300 amino acids) is non catalytically active, but sequence analysis has found a non-canonical PIWI-like motif (found in RNA splicing helicase Brr2) which is unable to bind DNA but is involved in displacement of RPA (Replication Protein A) from ssDNA 8. This study focussed on better understanding the role of this N-terminal domain of this helicase in vitro using BioID2. Biotin ligase protein interaction Identification generation 2 (BioID2) is a method for screening physiologically relevant protein interactions that occur in living cells originally developed by Roux et al.. BioID2 uses the promiscuous ability of Biotin ligase from A. aeolicus with a mutated catalytical domain (R40G) to biotinylate nearby proteins, these proteins can be pulled out of solution using streptavidin beads and protein identity discovered via LC-MS/MS. This method allows high throughput screening of the proteome surrounding HelQ and offers insight into potential DNA repair pathways involving HelQ.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:07:37.926.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jin Ke Michele Pan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-07 08:00:54	ID requested
⏵ 1	2025-05-06 13:07:38	announced

Pan JM, Betts H, Cubbon A, He L, Bolt EL, Soultanas P, The human HELQ helicase and XRN2 exoribonuclease cooperate in R-loop resolution. Open Biol, 15(2):240112(2025) [pubmed]

10.1098/rsob.240112;

submitter keyword: HelQ, XRN2, HelQ, LC-MS/MS

Panos Soultanas
contact affiliation	University of Nottingham and QMC
contact email	panos.soultanas@nottingham.ac.uk
lab head
Jin Ke Michele Pan
contact affiliation	University of Nottingham
contact email	jin.pan@nottingham.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD049246

PRIDE project URI

• PRIDE
  1. PXD049246
     1. Label: PRIDE project
     2. Name: HelQ-BioID2 experiment to discover novel protein interactants of HelQ