PXD049209-1

PXD049209 is an original dataset announced via ProteomeXchange.

Title	Circadian Control of the Response of Macrophages to Plasmodium spp.-Infected Red Blood Cells.
Description	In this study, we sought to determine whether the intensity of the immune response to Plasmodium spp., the parasite causing malaria, depends on time of infection. For this, we considered the known existence of a circadian clock in immune cells which regulates several aspects of the immune response. Experiments were performed using mouse bone marrow-derived macrophages (BMDMs) stimulated ex vivo with red blood cells infected with Plasmodium berghei ANKA (iRBCs) or uninfected RBCs. First, we identified that lysed iRBCs triggered an immune response in macrophages. Then, by stimulating at 4 different circadian time points (16 h, 22 h, 28 h or 34 h post-synchronization of the cells clock), rhythms in reactive oxygen species and cytokines/chemokines were found. Furthermore, the analysis of the macrophage proteome and phosphoproteome revealed global changes according to treatment (iRBC or RBC) and to the circadian time point. In summary, our findings showed that the circadian clock within macrophages determines the magnitude of immune response upon stimulation with iRBCs, along with changes of the cell proteome and phosphoproteome.
HostingRepository	PRIDE
AnnounceDate	2024-06-26
AnnouncementXML	Submission_2024-06-26_15:57:24.700.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vincent Richard
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-06 07:19:29	ID requested
⏵ 1	2024-06-26 15:57:25	announced
2	2024-10-22 06:47:33	announced	2024-10-22: Updated project metadata.

10.4049/immunohorizons.2400021;

Carvalho Cabral P, Richard VR, Borchers CH, Olivier M, Cermakian N, Circadian Control of the Response of Macrophages to Plasmodium Spp.-Infected Red Blood Cells. Immunohorizons, 8(6):442-456(2024) [pubmed]

submitter keyword: immune responses., circadian rhythms, infected red blood cells,Macrophages, Plasmodium spp.

Christoph Borchers
contact affiliation	Segal Cancer Proteomics Centre, Lady Davis Institute, Jewish General Hospital, McGill University, Montreal, QC, Canada. christoph.borchers@mcgill.ca
contact email	christoph.borchers@mcgill.ca
lab head
Vincent Richard
contact affiliation	Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University
contact email	vincentroyrichard@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD049209

PRIDE project URI

• PRIDE
  1. PXD049209
     1. Label: PRIDE project
     2. Name: Circadian Control of the Response of Macrophages to Plasmodium spp.-Infected Red Blood Cells.