PXD049202 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Automated preparation of plasma lipids, metabolites, and proteins for LC/MS-based analysis of a high-fat diet in mice |
| Description | Blood plasma is one of the most commonly analyzed and easily accessible biological samples. Here, we describe an automated liquid-liquid extraction (LLE) platform that generates accurate, precise, and reproducible samples for metabolomic, lipidomic, and proteomic analyses from a single aliquot of plasma while minimizing hands-on time and avoiding contamination from plasticware. We applied mass spectrometry to examine the metabolome, lipidome, and proteome of 90 plasma samples to determine the effects of age, time of day, and a high-fat diet in mice. From 25 μL of mouse plasma, we identified 907 lipid species from 16 different lipid classes and subclasses, 233 polar metabolites, and 344 proteins. We found that the high fat diet induced only mild changes in the polar metabolome, upregulated Apolipoproteins, and induced substantial shifts in the lipidome, including a significant increase in arachidonic acid (AA) and a decrease in eicosapentaenoic acid (EPA) content across all lipid classes. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-05-06 |
| AnnouncementXML | Submission_2025-05-06_12:55:57.454.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Ngoc Vu |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-02-06 06:39:33 | ID requested | |
| ⏵ 1 | 2025-05-06 12:55:58 | announced | |
Publication List
| 10.1016/j.jlr.2024.100607; |
| Vu N, Maile TM, Gollapudi S, Gaun A, Seitzer P, O'Brien JJ, Hackett SR, Zavala-Solorio J, McAllister FE, Kolumam G, Keyser R, Bennett BD, Automated preparation of plasma lipids, metabolites, and proteins for LC/MS-based analysis of a high-fat diet in mice. J Lipid Res, 65(9):100607(2024) [pubmed] |
Keyword List
| ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project, Plasma (B/D-HPP) |
| submitter keyword: multiomics, proteomics,mouse plasma |
Contact List
| Bryson Bennett |
|---|
| contact affiliation | Director of Metabolomics Calico Life Sciences LLC |
| contact email | bryson@calicolabs.com |
| lab head | |
| Ngoc Vu |
|---|
| contact affiliation | Calico Life Sciences |
| contact email | ngoc@calicolabs.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD049202 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD049202
- Label: PRIDE project
- Name: Automated preparation of plasma lipids, metabolites, and proteins for LC/MS-based analysis of a high-fat diet in mice
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