PXD049165-1

PXD049165 is an original dataset announced via ProteomeXchange.

Title	Flavobacterium T9SS Extended Translocon
Description	Secretion Systems are protein export machines that enable bacteria to exploit their environment through the release of protein effectors. The Type 9 Secretion System (T9SS) is responsible for protein export across the outer membrane (OM) of bacteria of the phylum Bacteroidota. Here we use deletion of the T9SS motor protein to trap the T9SS Flavobacterium johnsoniae in the process of substrate transport. CryoEM analysis of purified substrate-bound T9SS translocons revealed an extended translocon compared to previous analyses. The translocon core is augmented by a five-subunit periplasmic structure incorporating the proteins SprE, PorD, and a homologue of the canonical periplasmic chaperone Skp. Substrate proteins bind to the extracellular loops of a carrier protein within the translocon pore. As transport intermediates accumulate on the translocon when energetic input is removed, we deduce that release of the substrate-carrier protein complex from the translocon is the energy-requiring step in T9SS transport.
HostingRepository	PRIDE
AnnounceDate	2024-03-05
AnnouncementXML	Submission_2024-03-05_09:02:29.745.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD049165
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Frederic Lauber
SpeciesList	scientific name: Flavobacterium johnsoniae UW101; NCBI TaxID: 376686;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-04 22:57:11	ID requested
⏵ 1	2024-03-05 09:02:30	announced

10.6019/PXD049165;

submitter keyword: Bacteroidetes,Type 9 Secretion System

Ben C. Berks
contact affiliation	Department of Biochemistry, University of Oxford, Oxford, United Kingdom
contact email	ben.berks@bioch.ox.ac.uk
lab head
Frederic Lauber
contact affiliation	De Duve Institute - University of Louvain
contact email	frederic.lauber@uclouvain.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049165
     1. Label: PRIDE project
     2. Name: Flavobacterium T9SS Extended Translocon