PXD049149-1
PXD049149 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Site-selective photo crosslink to methyllysine readers via single electron transfer between sulfonium and tryptophan |
| Description | Reader proteins play significant roles in cell signaling from site-specific lysine methylation, but the progress of new reader investigations is rather slow. Photoreactive crosslinkers enable reader identifications, but it requires laborious chemical synthesis for optimization of the photoreactive group placement. In addition, active intermediate such as nitrene and carbene may cause significant non-specific crosslinking. Here we report dimethylsulfonium as methyllysine mimic binds to specific readers and subsequently crosslinks to conserved tryptophan inside binding pocket via single electron transfer under UV irradiation. Because the σ-π electron-donor-acceptor interaction between sulfonium and indole is a key step, the crosslinking exhibits excellent tryptophan site-selectivity and orthogonality to distinct methyllysine readers. Moreover, excited tryptophan without proximate sulfonium leads to minimal non-specific crosslinking from relaxation. Consequently, this method could escalate discovery of methyllysine readers from complicated cell samples. Furthermore, this photo crosslinking concept could be expanded to develop more types of microenvironment-dependent conjugations to site-specific tryptophan. |
| HostingRepository | iProX |
| AnnounceDate | 2024-02-04 |
| AnnouncementXML | Submission_2024-02-04_01:25:21.020.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Nan Zhao |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480; Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-02-04 01:24:53 | ID requested | |
| ⏵ 1 | 2024-02-04 01:25:21 | announced | |
| 2 | 2024-10-12 00:17:23 | announced | 2024-10-12: Update information. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: photo-crosslink, methyllysine, readers sulfonium |
Contact List
| Lihua Zhang | |
|---|---|
| contact affiliation | Dalian Institute of Chemical Physics |
| contact email | lihuazhang@dicp.ac.cn |
| lab head | |
| Nan Zhao | |
| contact affiliation | Dalian Institute of Chemical Physics |
| contact email | zhaonan@dicp.ac.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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