PXD049141-1

PXD049141 is an original dataset announced via ProteomeXchange.

Title	[HPP-2.0] Dynamic phosphotyrosine-dependent signaling profiling in living cells by two-dimensional proximity proteomics
Description	Tyrosine phosphorylation (pTyr)-dependent signaling pathways play vital role in various biological processes, which are spatiotemporally assembled and dynamically regulated on minute-scale by pTyr in living cells. Studying these pTyr-mediated signaling complexes is therefore challenging due to the highly dynamic nature of the protein complexes and the low abundance of pTyr. In this study, we adopted minute-resolution APEX2-based proximity labeling (PL) in living cells and Src SH2 superbinder-based pTyr peptide enrichment for simultaneously profiling these protein complexes and associated pTyr sites from the same affinity-purified sample. Upon different time-course of EGF stimulation of the living cells stably expressing APEX2-FLAG-GRB2, we constructed two-dimensional time-course curves for both interactome and tyrosine phosphoproteome.
HostingRepository	iProX
AnnounceDate	2024-02-03
AnnouncementXML	Submission_2024-02-03_19:46:35.807.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ruijun Tian
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-03 19:45:20	ID requested
⏵ 1	2024-02-03 19:46:36	announced

Dataset with its publication pending

submitter keyword: phosphotyrosine, living cells, proximity proteomics

Ruijun Tian
contact affiliation	Southern University of Science and Technology
contact email	tianrj@sustech.edu.cn
lab head
Ruijun Tian
contact affiliation	Southern University of Science and Technology
contact email	13667356941@163.com
dataset submitter

iProX dataset URI