PXD049137-1

PXD049137 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	CRISPR-assisted RNA-protein interaction detection (CARPID) combined with quantitative mass spectrometry was used to identify proteins interacting with the long non-coding RNA (lncRNA) Taurine Up-regulated 1 (TUG1) in HEK293T cells under physiological conditions and after Camptothecin (CPT) treatment.
Description	This project utilized CRISPR-assisted RNA-protein interaction detection (CARPID) combined with mass spectrometry to identify proteins interacting with the long non-coding RNA (lncRNA) TUG1 in HEK293T cells. TUG1 preferentially interacts with known R-loop-associated proteins, particularly under Camptothecin (CPT) treatment. Additionally, we identified interacting proteins for the lncRNAs MALAT1 to validate the binding specificity of TUG1.
HostingRepository	jPOST
AnnounceDate	2025-07-17
AnnouncementXML	Submission_2025-07-16_21:52:10.138.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Reiko Nakagawa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-03 17:51:03	ID requested
⏵ 1	2025-07-16 21:52:10	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Long non-coding RNA, TUG1, HEK293T, CARPID

submitter keyword: Long non-coding RNA, TUG1, HEK293T, CARPID

Contact List

Yutaka Kondo
lab head
Reiko Nakagawa
contact affiliation	RIKEN BDR
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002495/