PXD049136-2

PXD049136 is an original dataset announced via ProteomeXchange.

Title	Widespread prevalence of a post-translational modification in activation of an essential bacterial DNA damage response
Description	DNA methylation plays central roles in diverse cellular processes, ranging from error-correction during replication to regulation of bacterial defense mechanisms. Nevertheless, certain aberrant methylation modifications can have lethal consequences. The mechanisms by which bacteria detect and respond to such damage remain incompletely understood. Here, we discover a highly conserved but previously uncharacterized transcription factor (Cada2), which orchestrates a methylation-dependent adaptive response in Caulobacter. This response operates independently of the SOS response, governs the expression of genes crucial for direct repair, and is essential for surviving methylation-induced damage. Our molecular investigation of Cada2 reveals a cysteine methylation-dependent post-translational modification and mode of action distinct from its E. coli counterpart, a trait conserved across all bacteria harboring a Cada2-like homolog instead. Extending across the bacterial kingdom, our findings support the notion of divergence and co-evolution of adaptive response transcription factors and their corresponding sequence-specific DNA motifs. Despite this diversity, the ubiquitous prevalence of adaptive response regulators underscores the significance of a transcriptional switch, mediated by methylation post-translational modification, in driving a specific and essential bacterial DNA damage response.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:28:59.754.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aditya Kamat
SpeciesList	scientific name: Caulobacter crescentus (strain NA1000 / CB15N); NCBI TaxID: 565050;
ModificationList	monomethylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-03 08:38:15	ID requested
1	2024-02-09 08:31:41	announced
⏵ 2	2024-10-22 06:29:00	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: methylation damage, Adaptive response, DNA repair,DNA damage, bacteria, Caulobacter, SOS response, bacterial PTMs

Dr. Anjana Badrinarayanan
contact affiliation	National Centre for Biological Sciences (NCBS), Bangalore, India
contact email	anjana@ncbs.res.in
lab head
Aditya Kamat
contact affiliation	National Centre for Biological Sciences
contact email	adityak@ncbs.res.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049136
     1. Label: PRIDE project
     2. Name: Widespread prevalence of a post-translational modification in activation of an essential bacterial DNA damage response