PXD049117-1

PXD049117 is an original dataset announced via ProteomeXchange.

Title	TAF2 condensation in nuclear speckles links basal transcription factor TFIID to RNA splicing
Description	TFIID is an essential eukaryotic transcription factor, which is required for RNA polymerase II promoter recognition and activation. As a multiprotein complex, TFIID contains several intrinsically disordered regions (IDRs), but the functions of these IDRs are unknown. Here, we show that a conserved IDR drives the TFIID subunit TAF2 to nuclear speckles, where it forms biomolecular condensates, separate from the TFIID complex. Quantitative mass spectrometry analyses reveal that the TAF2 IDR is required for the interaction with the nuclear speckle and spliceosome-associated protein SRRM2, which is thereby recruited to TFIID. The formation of SRRM2-free TFIID complexes elicits a set of unique alternative splicing events. These include events in RNAs coding for proteins involved in transcription and transmembrane transport. Together, these data identify an IDR of the basal transcription machinery as a molecular switch between nuclear compartments to control protein complex composition and pre-mRNA splicing.
HostingRepository	PRIDE
AnnounceDate	2024-04-26
AnnouncementXML	Submission_2024-04-26_05:30:23.565.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tanja Bhuiyan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-02 06:30:28	ID requested
⏵ 1	2024-04-26 05:30:24	announced
2	2024-10-22 06:37:27	announced	2024-10-22: Updated project metadata.

10.1101/2024.02.05.578926;

submitter keyword: TAF2,Transcription, nuclear speckles, biomolecular condensates, TFIID, splicing, SRRM2, intrinsically disordered region, phase separation, nuclear bodies

Sebastian J. Arnold
contact affiliation	Institute of Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine, University of Freiburg, Albertstrasse 25, 79104 Freiburg, Germany.
contact email	sebastian.arnold@pharmakol.uni-freiburg.de
lab head
Tanja Bhuiyan
contact affiliation	University of Freiburg
contact email	tanja.bhuiyan@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD049117

PRIDE project URI

• PRIDE
  1. PXD049117
     1. Label: PRIDE project
     2. Name: TAF2 condensation in nuclear speckles links basal transcription factor TFIID to RNA splicing