PXD049116 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell function (part 6: Mammary Fibroblasts Proteome) |
Description | Intercellular communication between different cell types in solid tumors contributes to tumor growth and metastatic dissemination. The secretome of cancer-associated fibroblasts (CAFs) plays major roles in these processes. Using human mammary CAFs, we unveil a mechanism of cell-cell communication between CAFs with a myofibroblast phenotype and endothelial cells (ECs) based on intercellular protein transfer through extracellular vesicles (EVs). CAFs transfer proteins to ECs, including plasma membrane receptors, which we have identified by using mass spectrometry-based proteomics. Using THY1 as an example of transferred plasma membrane-bound protein, we show that CAF-derived proteins can influence how ECs interact with other cell types. Here, we show that CAFs produce high amounts of matrix-bound EVs that have a key role in protein transfer. Hence, our work paves the way for further studies to understand how CAF-derived matrix-bound EVs influence tumor pathology by regulating functions of neighboring cancer, stromal and immune cells. |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-16 |
AnnouncementXML | Submission_2024-02-16_08:23:20.785.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sergio Lilla |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-02-02 04:41:07 | ID requested | |
⏵ 1 | 2024-02-16 08:23:21 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: breast cancer,Cancer-associated fibroblasts, extracellular vesicles, endothelial cells, myCAFs, matrix-bound vesicles, intercellular protein transfer. |
Contact List
Sara Rossana |
contact affiliation | CRUK - Scotland Institute - Switchback Rd, Bearsden, Glasgow G61 1BD - United Kingdom |
contact email | s.zanivan@beatson.gla.ac.uk |
lab head | |
Sergio Lilla |
contact affiliation | Proteomics |
contact email | s.lilla@beatson.gla.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD049116 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD049116
- Label: PRIDE project
- Name: Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell function (part 6: Mammary Fibroblasts Proteome)