PXD049092-1

PXD049092 is an original dataset announced via ProteomeXchange.

Title	Roles of Mettl14 in mouse limb bud development
Description	m6A regulates virtually every step in RNA metabolism. However, its toles in limb development remains largely unknown. To understand the roles, we created a limb bud-specific conditional knockout (cKO) mice and control heterozygous (cHet) mice of the Mettl14 gene, which encodes an essential subunit in the m6A methyltransferase complex METTL3/METTL14. We harvested limb buds from the mice on E12.5 and applied the proteins to quantitative mass spectrometry to understand how the depletion of Mettl14 affected the proteomes.
HostingRepository	PRIDE
AnnounceDate	2025-04-03
AnnouncementXML	Submission_2025-04-03_10:34:00.979.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nobuaki Kikyo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-01 17:26:21	ID requested
⏵ 1	2025-04-03 10:34:01	announced

Dataset with its publication pending

submitter keyword: Mettl14,mouse, limb bud, quantitative mass spectrometry

Nobuaki Kikyo
contact affiliation	Stem Cell Institute, University of Minnesota, USA
contact email	kikyo001@umn.edu
lab head
Nobuaki Kikyo
contact affiliation	University of Minnesota
contact email	kikyo001@umn.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD049092

PRIDE project URI

• PRIDE
  1. PXD049092
     1. Label: PRIDE project
     2. Name: Roles of Mettl14 in mouse limb bud development