PXD049077-1

PXD049077 is an original dataset announced via ProteomeXchange.

Title	Identification of the most immunoreactive antigens of Candida auris to sera from infected mice
Description	Candida auris is a recently found pathogenic yeast that causes systemic infections, showing a high mortality rate. The delay on making a correct diagnosis of C. auris is a current problem in the healthcare system setting. As immunoproteomics studies are important to identify immunoreactive proteins for new diagnostic strategies, in this study immunocompetent murine systemic infections caused by non-aggregative and aggregative phenotypes of C. auris, and by Candida albicans and Candida haemulonii were carried out, and the obtained sera were used to study their immunoreactivity against C. auris proteins. The aim was to identify the most immunoreactive antigens of this yeast. Thirteen spots were recognized by sera from mice infected with both C. auris phenotypes and analyzed by mass spectrometry. They corresponded to enolase, phosphoglycerate kinase, glyceraldehyde 3-phosphate dehydrogenase and phosphoglycerate mutase. These four proteins seem to be also recognized by sera obtained from human patients with disseminated C. auris infection, but not by sera obtained from mice infected with other fungi such as C. albicans or Aspergillus fumigatus. In conclusion, this study showed that the identified proteins could be potential candidates to be studied as new diagnostic or even therapeutic targets for C. auris.
HostingRepository	PRIDE
AnnounceDate	2024-06-23
AnnouncementXML	Submission_2024-06-23_01:17:15.531.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aitziber Antoran
SpeciesList	scientific name: [Candida] auris; NCBI TaxID: 498019; scientific name: Saccharomycetales; NCBI TaxID: NCBITaxon:4892;
ModificationList	carbamoylated residue; monohydroxylated residue
Instrument	Q Exactive HF; Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-01 02:58:43	ID requested
⏵ 1	2024-06-23 01:17:16	announced

10.1021/acs.jproteome.3c00752;

Areitio M, Antoran A, Rodriguez-Erenaga O, Aparicio-Fernandez L, Martin-Souto L, Buldain I, Zaldibar B, Ruiz-Gaitan A, Pem, á, n J, Rementeria A, Ramirez-Garcia A, to IgGs from Systemic Infections in Mice. J Proteome Res, 23(5):1634-1648(2024) [pubmed]

submitter keyword: Candida auris, antigen, systemic infections, proteome, western blot

Andoni Ramirez
contact affiliation	Immunology, Microbiology and Parasitology Faculty of Science and Technology University of the Basque Country (UPV/EHU) Spain
contact email	andoni.ramirez@ehu.eus
lab head
Aitziber Antoran
contact affiliation	University of the Basque Country (UPV/EHU)
contact email	aitziber.antoran@ehu.eus
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049077
     1. Label: PRIDE project
     2. Name: Identification of the most immunoreactive antigens of Candida auris to sera from infected mice