PXD049074-2

PXD049074 is an original dataset announced via ProteomeXchange.

Title	Proteomic insight into arabinogalactan utilization by particle-associated Maribacter sp. MAR_2009_72
Description	Arabinose and galactose are major components of both particulate and dissolved organic matter. They often constitute polysaccharides such as arabinogalactan. Field studies have demonstrated a turnover of arabinogalactan, but enzymes and organisms involved have not been explored. In this study, the degradation of arabinogalactan by the marine flavobacterium Maribacter sp. MAR_2009_72 was investigated with growth experiments and proteomic analyses. Growth of Maribacter sp. MAR_2009_72 on arabinogalactan displayed its ability to utilize arabinogalactan. Proteomic analysis of cells grown on arabinogalactan, arabinose, galactose or glucose revealed expression of specific proteins in presence of arabinogalactan, mainly glycoside hydrolases. Candidates for extracellular glycan hydrolysis are five alpha-L-arabinofuranosidases affiliating with glycosyl hydrolase (GH) families 43 and 51, four unsaturated rhamnogalacturonyl hydrolases (GH105) and a protein with a glycosyl hydrolase family like domain. We detected expression of three induced TonB-dependent SusCD transporter systems, one SusC and nine glycosyl hydrolases with a predicted periplasmatic location. These are affiliated with the families GH3, GH10, GH29, GH31, GH67, GH78 and GH115. The genes are located within three canonical polysaccharide utilization loci (PUL), but also outside of the defined loci. PULs can be classified as specific for arabinogalactan, galactose-containing glycans and for arabinose-containing glycans. The breadth of enzymatic functions expressed in Maribacter sp. MAR_2009_72 as response to arabinogalactan from terrestrial plant larch suggests that Flavobacteriia are main catalysts of the rapid turnover of arabinogalactans in the marine environment.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:37:57.132.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD049074
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Saskia Kalenborn
SpeciesList	scientific name: Maribacter sp. MAR_2009_72; NCBI TaxID: 1250050;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-01 01:13:52	ID requested
1	2024-05-02 00:18:56	announced
⏵ 2	2024-10-22 06:37:57	announced	2024-10-22: Updated project metadata.

10.1093/FEMSEC/FIAE045;

10.6019/PXD049074;

submitter keyword: Flavobacteriia

Jens Harder
contact affiliation	Department of Molecular Ecology MPI for Marine Microbiology Celsiusstr. 1 D-28359 Bremen Germany
contact email	jharder@mpi-bremen.de
lab head
Saskia Kalenborn
contact affiliation	Max Planck Institute for marine Microbiology
contact email	skalenbo@mpi-bremen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049074
     1. Label: PRIDE project
     2. Name: Proteomic insight into arabinogalactan utilization by particle-associated Maribacter sp. MAR_2009_72