PXD049039-1

PXD049039 is an original dataset announced via ProteomeXchange.

Title	Genes for laminarin degradation are dispersed in the genomes of particle-associated Maribacter so. MAR_2009_72
Description	Laminarin is a cytosolic storage polysaccharide of phytoplankton and macroalgae and accounts for over 10% of the world´s annually fixed carbon dioxide. Algal disruption, e.g., by viral lysis releases laminarin. The soluble sugar is rapidly utilized by free-living planktonic bacteria, in which sugar transporters and the degrading enzymes are frequently encoded in polysaccharide utilization loci. The annotation of flavobacterial genomes failed to identify canonical laminarin utilization loci in several particle-associated bacteria, in particular in strains of Maribacter. In this study, we report in vivo utilization of laminarin by Maribacter forsetii accompanied by additional cell growth and proliferation. Laminarin utilization coincided with the induction of an extracellular endo-laminarinase, SusCD outer membrane oligosaccharide transporters and a periplasmic glycosyl hydrolase family 3 protein. ABC transporter and sugar kinases were expressed. Endo-laminarinase activity was also observed in Maribacter sp. MAR_2009_72, Maribacter sp. Hel_I_7 and Maribacter dokdonensis MAR_2009_60. Maribacter dokdonensis MAR_2009_71 lacked the large endo-laminarinase gene in the genome and had no endo-laminarinase activity. In all genomes, genes of induced proteins were scattered across the genome rather than clustered in a laminarin utilization locus. These observations revealed that the Maribacter strains investigated in this study participate in laminarin utilization, but in contrast to many free-living bacteria, there is no co localisation of genes encoding the enzymatic machinery for laminarin utilization.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_02:47:08.789.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD049039
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Saskia Kalenborn
SpeciesList	scientific name: Maribacter sp. MAR_2009_72; NCBI TaxID: 1250050;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-31 05:44:45	ID requested
⏵ 1	2024-10-17 02:47:09	announced

Kalenborn S, Z, ü, hlke D, Reintjes G, Riedel K, Amann RI, Harder J, species. Front Microbiol, 15():1393588(2024) [pubmed]

10.3389/fmicb.2024.1393588;

10.6019/PXD049039;

submitter keyword: Flavobacteriia

Jens Harder
contact affiliation	Department of Molecular Ecology MPI for Marine Microbiology Celsiusstr. 1 D-28359 Bremen Germany
contact email	jharder@mpi-bremen.de
lab head
Saskia Kalenborn
contact affiliation	Max Planck Institute for marine Microbiology
contact email	skalenbo@mpi-bremen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049039
     1. Label: PRIDE project
     2. Name: Genes for laminarin degradation are dispersed in the genomes of particle-associated Maribacter so. MAR_2009_72