PXD049037-1

PXD049037 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mass Spectometry analysis of the DnaK clients
Description	A co-affinity assay coupled to downstream LC-MS analyses were used to investigate the effect of linker mutations on the DnaK interactome as previously described3. The purified recombinant forms of DnaK and DnaKLS were immobilized (as bait) by His-tag affinity on HisPur cobalt resin suspended in TBS (10 mM Tris-HCl pH 7.5, 50 mM NaCl and 10 Mm imidazole) and incubated at 4 °C for 4 hours with gently rocking motion to allow maximum binding.
HostingRepository	jPOST
AnnounceDate	2025-03-28
AnnouncementXML	Submission_2025-03-27_08:00:04.906.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TAWANDA ZININGA
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; S-carboxymethyl-L-cysteine; L-methionine sulfoxide; 2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln)
Instrument	6520A Quadrupole Time-of-Flight LC/MS

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-31 03:50:45	ID requested
⏵ 1	2025-03-27 08:00:05	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: E. coli, DnaK, Linker switch mutants

submitter keyword: E. coli, DnaK, Linker switch mutants

Contact List

Tawanda Zininga
lab head
TAWANDA ZININGA
contact affiliation	Stellenbosch University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002494/