PXD049003-1

PXD049003 is an original dataset announced via ProteomeXchange.

Title	Deciphering Staphylococcus aureus-host dynamics using dual Activity-based protein profiling of ATP-interacting proteins
Description	This study focuses on ATP-binding proteins to dissect the complex interplay between Staphylococcus aureus and human cells, particularly macrophages (THP-1) and keratinocytes (HaCaT), during the course of an intracellular infection. A snapshot of the various protein activity and function is provided using a desthiobiotin-ATP probe, which targets ATP-interacting proteins. In S. aureus, we observe enrichment in pathways required for nutrient acquisition, biosynthesis and metabolism of amino acids and energy metabolism when located inside human cells. Additionally, the direct profiling of the protein activity revealed specific adaptations of S. aureus to the keratinocytes and macrophages. Mapping the differentially activated proteins to biochemical pathways in the human cells with intracellular bacteria revealed cell-type specific adaptations to bacterial challenges where THP-1 cells prioritized immune defenses, autophagic cell death, and inflammation. In contrast, HaCaT cells emphasized barrier integrity and immune activation. We also observe bacterial modulation of host processes and metabolic shifts.
HostingRepository	PRIDE
AnnounceDate	2024-04-22
AnnouncementXML	Submission_2024-04-22_00:08:13.416.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephen Dela Ahator
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-30 04:40:42	ID requested
⏵ 1	2024-04-22 00:08:13	announced
2	2024-10-22 06:36:57	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: ATP-interacting proteins, THP-1 cell infection, Staphylococcus aureus, Activity-Based protein profiling, HaCaT cel infectionl,Host-pathogen interaction

Mona Johannessen
contact affiliation	1Centre for New Antibacterial Strategies (CANS) & Research Group for Host-Microbe Interactions, Department of Medical Biology, Faculty of Health Sciences, UiT- The Arctic University of Norway, 9037 Tromsø
contact email	mona.johannessen@uit.no
lab head
Stephen Dela Ahator
contact affiliation	Arctic University of Norway, UiT
contact email	delaahator@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD049003

PRIDE project URI

• PRIDE
  1. PXD049003
     1. Label: PRIDE project
     2. Name: Deciphering Staphylococcus aureus-host dynamics using dual Activity-based protein profiling of ATP-interacting proteins