PXD048990-1

PXD048990 is an original dataset announced via ProteomeXchange.

Title	Morphophysiological and Proteomic Profiling Reveals Mechanisms Underlying Nitrogen Use Efficiency in Popcorn (Zea mays everta)
Description	Nitrogen (N) is an essential macronutrient for agricultural production, and its excessive use causes not only economic but also environmental damage. However, improving nitrogen use efficiency (NUE) remains a complex task for achieving more productive crops under conditions of limited nitrogen availability. To the best of our knowledge, this is the first study aiming to integrate the morphophysiological responses and proteomic profiles of leaves and roots of popcorn (Zea mays everta). For this, two inbred lines contrasting for NUE, i.e., P2 (high NUE) and L80 (low NUE), were cultivated under high (100% of N supply) and low (10% of N supply) nitrogen conditions. Morphological and physiological traiits such as photochemical quenching (qP), non-photochemical quenching (NPQ), quantum yield of photosystem II (ΦPSII), and potential photosynthesis (Apot) were evaluated. P2 showed more pronounced vegetative growth under low N, as well as higher values of qP, NPQ, and ΦPSII. Comparative proteomic analysis of the leaves identified 215 differentially accumulated proteins (DAPs) in P2 and 168 DAPs in L80, while in roots, 127 DAPs were observed in P2 and 172 in L80. In leaves, DAPs involved in the response to oxidative stress, energy metabolism, and photosynthesis represented the main differences between P2 and L80. In roots, DAPs involved in nitrate transport, ammonium assimilation, and amino acid metabolism seem to have contributed to the improved NUE in P2. This study provides valuable insights into the molecular mechanisms underlying NUE and opens avenues for molecular breeding aimed at selecting superior genotypes for the development of a more sustainable agriculture.
HostingRepository	PRIDE
AnnounceDate	2025-03-11
AnnouncementXML	Submission_2025-03-11_13:54:24.242.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vitor Pinto
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-29 18:16:12	ID requested
⏵ 1	2025-03-11 13:54:24	announced

10.1016/J.PLAPHY.2025.109581;

submitter keyword: Abiotic stress. Comparative proteomics. Leaf Proteomics. Root proteomics. Leaf fluorescence. Photosynthesis

Vitor Batista Pinto
contact affiliation	Laboratório de Biologia Celular e Tecidual - Centro de Biociências e Biotecnologia - UENF
contact email	vitorbp@uenf.br
lab head
Vitor Pinto
contact affiliation	Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)
contact email	vitorbp@uenf.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048990
     1. Label: PRIDE project
     2. Name: Morphophysiological and Proteomic Profiling Reveals Mechanisms Underlying Nitrogen Use Efficiency in Popcorn (Zea mays everta)