PXD048981-1

PXD048981 is an original dataset announced via ProteomeXchange.

Title	Lack of phosphatidylinositol 3-kinase VPS34 in regulatory T cells leads to a fatal lymphoproliferative disorder without affecting their development
Description	Regulatory T (Treg) cells are essential for the maintenance of immunological tolerance, yet the molecular components required for their maintenance and effector functions remain incompletely defined. Inactivation of VPS34 in Treg cells led to an early, lethal phenotype, with massive effector T cell activation and inflammation, like mice lacking Treg cells completely. However, VPS34-deficient Treg cells developed normally, populated the peripheral lymphoid organs and effectively supressed conventional T cells in vitro. Our data suggest that VPS34 is required for the maturation of Treg cells or that mature Treg cells depend on VPS34 for survival. Functionally, we observed that lack of VPS34 activity impairs cargo processing upon transendocytosis, that defective autophagy contributes to, but is not sufficient to explain this lethal phenotype, and that loss of VPS34 activity induces a state of heightened metabolic activity that may interfere with metabolic networks required for maintenance or suppressive functions of Treg cells.
HostingRepository	PRIDE
AnnounceDate	2024-01-29
AnnouncementXML	Submission_2024-01-29_10:53:09.416.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alejandro Brenes
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-29 10:21:34	ID requested
⏵ 1	2024-01-29 10:53:09	announced
2	2024-10-22 06:28:01	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: mouse, VPS34, PI3K, T cells, T regs

Doreen Cantrell
contact affiliation	Division of Cell Signalling & Immunology, University of Dundee
contact email	d.a.cantrell@dundee.ac.uk
lab head
Alejandro Brenes
contact affiliation	Cell Signalling & Immunology, University of Dundee
contact email	ajbrenesmurillo@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048981
     1. Label: PRIDE project
     2. Name: Lack of phosphatidylinositol 3-kinase VPS34 in regulatory T cells leads to a fatal lymphoproliferative disorder without affecting their development