PXD048980-2
PXD048980 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | - Identification of Gαi2-interacting proteins via affinity pull-down and quantitative mass-spectrometry analysis. |
Description | In studying patients with activating GNAI2 mutations, we observed T cell hyperresponsiveness that was independent of cAMP suppression. Therefore, we hypothesized that Gαi2 utilizes alternative signal transduction pathways which might be delineated by identifying its interacting proteins in T cells. To accomplish this, we performed affinity pulldown followed by quantitative mass-spectrometry analysis. Using this approach, we identified both well-known and previously reported interactors of Gαi2 as well as 128 previously unreported interactors including RASA2. We validated RASA2, a GTPase-activating protein for RAS, and PP2A-A/C as Gαi2 effector targets by several methods: overexpression-coimmunoprecipitations using 293T cells, endogenous coimmunoprecipitations from patient T cells, and by mixing-pulldown experiments using purified recombinant proteins. Additional imaging experiments confirmed Gαi2-RASA2 interactions within living cells and delineated that activating mutations of Gαi2 sequester RASA2 toward the plasma membrane to promote RAS activation for T cell growth and proliferation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:59:16.768.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD048980 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | David Anderson |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-01-29 09:24:35 | ID requested | |
1 | 2024-09-20 05:01:00 | announced | |
⏵ 2 | 2024-10-22 06:59:17 | announced | 2024-10-22: Updated project metadata. |
Publication List
Ham H, Jing H, Lamborn IT, Kober MM, Koval A, Berchiche YA, Anderson DE, Druey KM, Mandl JN, Isidor B, Ferreira CR, Freeman AF, Ganesan S, Karsak M, Mustillo PJ, Teo J, Zolkipli-Cunningham Z, Chatron N, Lecoquierre F, Oler AJ, Schmid JP, Kuhns DB, Xu X, Hauck F, Al-Herz W, Wagner M, Terhal PA, Muurinen M, Barlogis V, Cruz P, Danielson J, Stewart H, Loid P, Rading S, Keren B, Pfundt R, Zarember KA, Vill K, Potocki L, Olivier KN, Lesca G, Faivre L, Wong M, Puel A, Chou J, Tusseau M, Moutsopoulos NM, Matthews HF, Simons C, Taft RJ, Soldatos A, Masle-Farquhar E, Pittaluga S, Brink R, Fink DL, Kong HH, Kabat J, Kim WS, Bierhals T, Meguro K, Hsu AP, Gu J, Stoddard J, Banos-Pinero B, Slack M, Trivellin G, Mazel B, Soomann M, Li S, Watts VJ, Stratakis CA, Rodriguez-Quevedo MF, Bruel AL, Lipsanen-Nyman M, Saultier P, Jain R, Lehalle D, Torres D, Sullivan KE, Barbarot S, Neu A, Duffourd Y, Similuk M, McWalter K, Blanc P, B, é, zieau S, Jin T, Geha RS, Casanova JL, Makitie OM, Kubisch C, Edery P, Christodoulou J, Germain RN, Goodnow CC, Sakmar TP, Billadeau DD, K, ü, ry S, Katanaev VL, Zhang Y, Lenardo MJ, Su HC, Germline mutations in a G protein identify signaling cross-talk in T cells. Science, 385(6715):eadd8947(2024) [pubmed] |
10.1126/science.add8947; |
10.6019/PXD048980; |
Keyword List
submitter keyword: GPCRs, G-protein coupled receptors, GNAI2,- Gαi2, G-protein |
Contact List
Helen C. Su | |
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contact affiliation | Human Immunological Diseases Section, Laboratory of Clinical Immunology and Microbiology, Division of Intramural Research, NIAID, NIH, Department of Health and Human Services, Unites States Government |
contact email | hsu@niaid.nih.gov |
lab head | |
David Anderson | |
contact affiliation | NIH/NIDDK |
contact email | davida@mail.nih.gov |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD048980 |
PRIDE project URI |
Repository Record List
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