PXD048976 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Structural basis of chloroplast transcription |
Description | Chloroplasts contain a dedicated genome, which encodes subunits of the photosynthesis machinery. Transcription of photosynthesis genes is predominantly carried out by a plastid-encoded RNA polymerase (PEP), a nearly 1 MDa complex composed of core subunits with homology to eubacterial RNA polymerases (RNAPs) and at least 12 additional chloroplast-specific PEP-associated proteins (PAPs). However, the architecture of this complex and the functions of the PAPs remain unknown. In this work, a 19-subunit PEP complex from Sinapis alba was purified by glycerol gradient centrifugation. All known subunits of the complex could be identified by LC-MS and the structure of the complex was determined by cryo-electron microscopy. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:31:05.158.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Noah Ditz |
SpeciesList | scientific name: Sinapis alba; NCBI TaxID: 3728; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-01-29 07:15:47 | ID requested | |
1 | 2024-02-19 02:36:08 | announced | |
⏵ 2 | 2024-10-22 06:31:05 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Plastid Encoded RNA Polymerase, Sinapis alba, PEP complex, Mustard |
Contact List
Hans-Peter Braun |
contact affiliation | Department of Plant Proteomics, Leibniz University Hannover |
contact email | braun@genetik.uni-hannover.de |
lab head | |
Noah Ditz |
contact affiliation | Department of plant proteomics, Leibniz Univerity Hannover |
contact email | ditz@genetik.uni-hannover.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048976
- Label: PRIDE project
- Name: Structural basis of chloroplast transcription