PXD048976

PXD048976 is an original dataset announced via ProteomeXchange.

Title	Structural basis of chloroplast transcription
Description	Chloroplasts contain a dedicated genome, which encodes subunits of the photosynthesis machinery. Transcription of photosynthesis genes is predominantly carried out by a plastid-encoded RNA polymerase (PEP), a nearly 1 MDa complex composed of core subunits with homology to eubacterial RNA polymerases (RNAPs) and at least 12 additional chloroplast-specific PEP-associated proteins (PAPs). However, the architecture of this complex and the functions of the PAPs remain unknown. In this work, a 19-subunit PEP complex from Sinapis alba was purified by glycerol gradient centrifugation. All known subunits of the complex could be identified by LC-MS and the structure of the complex was determined by cryo-electron microscopy.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:31:05.158.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Noah Ditz
SpeciesList	scientific name: Sinapis alba; NCBI TaxID: 3728;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-29 07:15:47	ID requested
1	2024-02-19 02:36:08	announced
⏵ 2	2024-10-22 06:31:05	announced	2024-10-22: Updated project metadata.

10.1016/J.MOLCEL.2024.02.003;

submitter keyword: Plastid Encoded RNA Polymerase, Sinapis alba, PEP complex, Mustard

Hans-Peter Braun
contact affiliation	Department of Plant Proteomics, Leibniz University Hannover
contact email	braun@genetik.uni-hannover.de
lab head
Noah Ditz
contact affiliation	Department of plant proteomics, Leibniz Univerity Hannover
contact email	ditz@genetik.uni-hannover.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048976
     1. Label: PRIDE project
     2. Name: Structural basis of chloroplast transcription