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PXD048976 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleStructural basis of chloroplast transcription
DescriptionChloroplasts contain a dedicated genome, which encodes subunits of the photosynthesis machinery. Transcription of photosynthesis genes is predominantly carried out by a plastid-encoded RNA polymerase (PEP), a nearly 1 MDa complex composed of core subunits with homology to eubacterial RNA polymerases (RNAPs) and at least 12 additional chloroplast-specific PEP-associated proteins (PAPs). However, the architecture of this complex and the functions of the PAPs remain unknown. In this work, a 19-subunit PEP complex from Sinapis alba was purified by glycerol gradient centrifugation. All known subunits of the complex could be identified by LC-MS and the structure of the complex was determined by cryo-electron microscopy.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNoah Ditz
SpeciesList scientific name: Sinapis alba; NCBI TaxID: 3728;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-01-29 07:15:47ID requested
12024-02-19 02:36:08announced
Publication List
Keyword List
submitter keyword: Plastid Encoded RNA Polymerase, Sinapis alba, PEP complex, Mustard
Contact List
Hans-Peter Braun
contact affiliationDepartment of Plant Proteomics, Leibniz University Hannover
contact emailbraun@genetik.uni-hannover.de
lab head
Noah Ditz
contact affiliationDepartment of plant proteomics, Leibniz Univerity Hannover
contact emailditz@genetik.uni-hannover.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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