PXD048968-2

PXD048968 is an original dataset announced via ProteomeXchange.

Title	A multi-omics approach to elucidate okadaic acid-induced changes in human HepaRG hepatocarcinoma cells
Description	Okadaic acid (OA) is a marine biotoxin that can induce diarrheic shellfish poisoning upon consumption. It can accumulate in filter-feeding shellfish, thereby reaching the human food chain. In addition to inducing severe gastrointestinal symptoms, OA can pass the intestinal barrier into the bloodstream, thereby reaching the liver. However, the effects of OA on the liver have not been much in the focus of research so far. Therefore, this study now focuses on elucidating changes in the proteome of HepaRG cells induced by OA. Furthermore, as a potent phosphatase inhibitor, we expected changes in the phosphoproteome of the cells. Therefore, we further investigated the phosphoproteome of the HepaRG cells after OA exposure. Results revealed changes in enzyme activity regulation, cytoskeleton organization and energy metabolism.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:43:55.174.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leonie Würger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-29 04:19:46	ID requested
1	2024-06-06 02:56:19	announced
⏵ 2	2024-10-22 06:43:56	announced	2024-10-22: Updated project metadata.

10.1007/s00204-024-03796-1;

Wuerger LTD, Sprenger H, Krasikova K, Templin M, Stahl A, Herfurth UM, Sieg H, Braeuning A, A multi-omics approach to elucidate okadaic acid-induced changes in human HepaRG hepatocarcinoma cells. Arch Toxicol, 98(9):2919-2935(2024) [pubmed]

submitter keyword: Liver Proteome, HepaRG Cells, Muli-omics,Okadaic Acid, Phosphoproteome

Albert Braeuning
contact affiliation	German Federal Institute for Risk Assessment
contact email	albert.braeuning@bfr.bund.de
lab head
Leonie Würger
contact affiliation	German Federal Institute for Risk Assessment
contact email	leonie.wuerger@bfr.bund.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD048968

PRIDE project URI

• PRIDE
  1. PXD048968
     1. Label: PRIDE project
     2. Name: A multi-omics approach to elucidate okadaic acid-induced changes in human HepaRG hepatocarcinoma cells