PXD048937-1

PXD048937 is an original dataset announced via ProteomeXchange.

Title	Using proximity labeling by turboID (biotin ligase) fused with ACSS2 combined with LC-MS for identification of the proteins associated with ACSS2 in oncogene RAS activation induced senescent human IMR90 cell
Description	Senescence-associated secretory phenotype (SASP), an established feature of cellular senescence, mediates the biological impacts of senescent cells on tissue microenvironments and contributes to ageing-associated disease progression. The metabolic enzyme ACSS2 produces acetyl-coA from acetate and epigenetically regulates gene expression mostly through histone acetylation. Whether and how ACSS2 regulates cellular senescence through non-histone acetylation mechanisms remain unclear. We used proximity labeling by turboID combined with LC-MS, identified PAICS, a key enzyme for purine metabolism which interacts with ACSS2. ACSS2 facilitates the acetylation of PAICS and promotes autophagy-mediated degradation of PAICS to limit purine metabolism and reduces dNTP pools for DNA damage repair, which results in cytoplasmic chromatin fragment (CCF) accumulation and SASP.
HostingRepository	PRIDE
AnnounceDate	2025-02-13
AnnouncementXML	Submission_2025-02-12_17:49:33.555.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yang Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-27 23:28:35	ID requested
⏵ 1	2025-02-12 17:49:33	announced

Dataset with its publication pending

submitter keyword: Human, IMR90, LC-MS

Pingyu Liu
contact affiliation	Human Phenome Institute,Zhangjiang FudanInternational Innovation Center, Fudan UniversityShanghai, 201203, China.
contact email	pingyu_liu@fudan.edu.cn
lab head
Yang Li
contact affiliation	fudan university
contact email	yangleduo1998@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD048937

PRIDE project URI

• PRIDE
  1. PXD048937
     1. Label: PRIDE project
     2. Name: Using proximity labeling by turboID (biotin ligase) fused with ACSS2 combined with LC-MS for identification of the proteins associated with ACSS2 in oncogene RAS activation induced senescent human IMR90 cell