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PXD048917-1

PXD048917 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOne-step N-terminomics based on isolation of protein N-terminal peptides from LysargiNase digests by tip-based strong cation exchange chromatography
DescriptionLarge-scale isolation of protein N-terminal peptides from LysargiNase digests was achieved by one-step strong cation exchange chromatography with disposable and parallel-processable pipette-tip columns. Novel cleavage sites for signal or transit peptides and non-canonical translation initiation sites were identified by the tip-based method. Together with two other methods similarly consisting of protease digestion and one-step tip LC, we have established a simple and comprehensive N- and C-terminomics approach called CHAMP (chromatographic amplification of protein terminal peptides).
HostingRepositoryjPOST
AnnounceDate2024-08-13
AnnouncementXMLSubmission_2024-08-12_18:03:43.446.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKazuya Morikawa
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; Acetyl
Instrumentinstrument; instrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-01-26 03:07:54ID requested
12024-08-12 18:03:44announced
Publication List
Morikawa K, Nishida H, Imami K, Ishihama Y, One-step N-Terminomics Based on Isolation of Protein N-Terminal Peptides From LysargiNase Digests by Tip-Based Strong Cation Exchange Chromatography. Mol Cell Proteomics, 23(9):100820(2024) [pubmed]
Keyword List
submitter keyword: N-terminomics, LysargiNase, SCX, CHAMP, HEK293T
Contact List
Yasushi Ishihama
lab head
Kazuya Morikawa
contact affiliationKyoto University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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