PXD048917-1

PXD048917 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	One-step N-terminomics based on isolation of protein N-terminal peptides from LysargiNase digests by tip-based strong cation exchange chromatography
Description	Large-scale isolation of protein N-terminal peptides from LysargiNase digests was achieved by one-step strong cation exchange chromatography with disposable and parallel-processable pipette-tip columns. Novel cleavage sites for signal or transit peptides and non-canonical translation initiation sites were identified by the tip-based method. Together with two other methods similarly consisting of protease digestion and one-step tip LC, we have established a simple and comprehensive N- and C-terminomics approach called CHAMP (chromatographic amplification of protein terminal peptides).
HostingRepository	jPOST
AnnounceDate	2024-08-13
AnnouncementXML	Submission_2024-08-12_18:03:43.446.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kazuya Morikawa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; Acetyl
Instrument	instrument; instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-26 03:07:54	ID requested
⏵ 1	2024-08-12 18:03:44	announced

Publication List

Morikawa K, Nishida H, Imami K, Ishihama Y, One-step N-Terminomics Based on Isolation of Protein N-Terminal Peptides From LysargiNase Digests by Tip-Based Strong Cation Exchange Chromatography. Mol Cell Proteomics, 23(9):100820(2024) [pubmed]

Morikawa K, Nishida H, Imami K, Ishihama Y, One-step N-Terminomics Based on Isolation of Protein N-Terminal Peptides From LysargiNase Digests by Tip-Based Strong Cation Exchange Chromatography. Mol Cell Proteomics, 23(9):100820(2024) [pubmed]

Keyword List

submitter keyword: N-terminomics, LysargiNase, SCX, CHAMP, HEK293T

submitter keyword: N-terminomics, LysargiNase, SCX, CHAMP, HEK293T

Contact List

Yasushi Ishihama
lab head
Kazuya Morikawa
contact affiliation	Kyoto University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002344/