PXD048872-2

PXD048872 is an original dataset announced via ProteomeXchange.

Title	Reversible S-Nitrosylation of bZIP67-Prx IIE Complex by Nitric Oxide Modulates Plant Lipid Content
Description	Nitric oxide (NO) is a key gasotransmitter that participates in a broad range of mechanisms controlling plant development and stress conditions. However, little is known about the specific role of this signaling molecule during lipid storage in the seeds. Here, we show NO is accumulated in developing embryos and regulates the fatty acid profile through the stabilization of the basic/leucine zipper transcription factor bZIP67. bZIP67 is a target of NO and nitro-linolenic acid that accumulates to induce the expression of the FAD3 desaturase, which is misregulated in a non-nitrosylable version of the protein. The post-translational modification of bZIP67 is reversed by trans-denitrosylation activity of Peroxiredoxin IIE, defining a feedback mechanism for bZIP redox regulation. These findings led us to provide a molecular framework for the control of seed fatty acid profile cuased by NO, and evidence regarding the in vivo functionality of nitro-fatty acids in plant developmental signaling. To identify new bZIP67 interactors, we performed an in vivo GFP pull-down assay using developing siliques of pbZIP67:GFP-bZIP67 and wild-type Col-0 as control.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:32:43.665.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominique Eeckhout
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-25 04:14:50	ID requested
1	2024-03-11 01:23:06	announced
⏵ 2	2024-10-22 06:32:44	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: AP-MS, fatty acid, nitric oxide,Arabidopsis, bZIP67

Bert De Rybel
contact affiliation	Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium VIB Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium
contact email	bert.derybel@psb.vib-ugent.be
lab head
Dominique Eeckhout
contact affiliation	Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, B-9052, Belgium VIB Center for Plant Systems Biology, Ghent, B-9052, Belgium
contact email	Dominique.Eeckhout@psb.ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD048872

PRIDE project URI

• PRIDE
  1. PXD048872
     1. Label: PRIDE project
     2. Name: Reversible S-Nitrosylation of bZIP67-Prx IIE Complex by Nitric Oxide Modulates Plant Lipid Content