PXD048861-1

PXD048861 is an original dataset announced via ProteomeXchange.

Title	GID/CTLH E3 ligase complex control cell fate programs for sexual development of Plasmodium falciparum
Description	Transmission of the malaria parasite Plasmodium falciparum requires the formation of specialised sexual cells called gametocytes. A hallmark of P. falciparum gametocyte development is its long duration, during which the parasite undergoes dramatic cellular remodelling including morphological, physiological and metabolic changes which result in the formation of a transmission ready, stage V gametocyte. Here we show that the PfGID E3 ubiquitin ligase complex regulates critical gametocyte cell fate programs through the targeted ubiquitination of key proteins. Deletion of PfGID complex components leads to an arrest in gametocyte development and a loss of transmission to mosquitoes. PfGID governs gametocyte development by fine-tuning the protein levels of two substrates: the ZFP36 family RNA binding protein GD1, and PfDPL, a cryptochrome-like protein. Our findings reveal that PfDPL regulates the expression of male-specific proteins early in gametocyte development that are essential for gametogenesis. In parallel to the PfDPL controlled cell fate program the RNA binding protein GD1 regulates transcripts crucial for gametocyte development by holding them in a state of translational repression. These findings illuminate the intricate molecular choreography underlying Plasmodium sexual development and provide insights into how single-celled eukaryotes execute cell-fate programs to navigate complex life cycles and adapt to diverse host environments.
HostingRepository	PRIDE
AnnounceDate	2026-01-25
AnnouncementXML	Submission_2026-01-25_09:26:46.866.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Dagley
SpeciesList	scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: NEWT:36329;
ModificationList	ubiquitination signature dipeptidyl lysine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; timsTOF Pro; Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-24 21:01:13	ID requested
⏵ 1	2026-01-25 09:26:47	announced

Dataset with its publication pending

submitter keyword: None

Alan Cowman
contact affiliation	The Walter and Eliza Hall institute of Medical Research, Department of Medical Biology, Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne
contact email	cowman@wehi.edu.au
lab head
Laura Dagley
contact affiliation	WEHI
contact email	dagley.l@wehi.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD048861

PRIDE project URI

• PRIDE
  1. PXD048861
     1. Label: PRIDE project
     2. Name: GID/CTLH E3 ligase complex control cell fate programs for sexual development of Plasmodium falciparum