PXD048855-1

PXD048855 is an original dataset announced via ProteomeXchange.

Title	Unravelling Intracellular Protein Corona Components of Nanoplas-tics via Photocatalytic Protein Proximity Labeling
Description	Bioaccumulation of nanoplastic particles draws increasing attention regarding environmental sustainability and biosafety. How nanoplastic particles interact with cellular milieu still remains elusive. Herein, we exemplify a general approach to profile the composition of “protein corona” interacting with nanoparticles via the photocatalytic protein proximity labeling method. To enable photocatalytic proximity labeling of proteome interacting with particles, iodine substituted BODIPY (I-BODIPY) is selected as the photosensitizer and covalently conjugated onto amino-polystyrene nanoparticle as a model system. Next, selec-tive proximity labeling of interacting proteins is demonstrated using I-BODIPY labeled nanoplastic particles in both E. coli lysate and live AML 12 cells. Mechanistic studies reveal the covalent modifications of proteins by amino-alkyne substrate is conducted via reactive oxygen species (ROS) photosensitization pathway. Further proteomic analysis uncovers that mitochon-dria-related proteins are intensively involved in protein corona, indicating substantial interactions between nanoplastic particles and mitochondria. In addition, proteostasis network components are also identified accompanied with consequent cellular pro-teome aggregation confirmed by fluorescence imaging. Together, this work exemplifies a general strategy to interrogate the composition of protein corona of nanomaterials by grafting them photo-oxidation property to enable photocatalytic protein proximity labeling function.
HostingRepository	iProX
AnnounceDate	2024-01-24
AnnouncementXML	Submission_2024-01-24_18:39:12.843.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhenduo Zhang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-24 18:38:49	ID requested
⏵ 1	2024-01-24 18:39:13	announced

Dataset with its publication pending

submitter keyword: protein corona, nanoplastic, proximity labeling, proteomics, mitochondria.

Zhenduo Zhang
contact affiliation	Dalian Institute of Chemical Physics
contact email	17615003187@163.com
lab head
Zhenduo Zhang
contact affiliation	Dalian Institute of Chemical Physics
contact email	17615003187@163.com
dataset submitter

iProX dataset URI