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PXD048849-1

PXD048849 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDetection of structure-specific interactions of Rab GTPases
DescriptionProtein–protein interactions (PPIs) mediate multiple essential functions and regulatory events in living organisms. The physical interactome of a given protein can be abnormally altered in response to external and internal cues, thus modulating cell physiology and contributing to human pathologies including neurodegenerative diseases. Despite substantial efforts, current approaches cannot pinpoint structure-specific PPIs or their interaction interfaces on a proteome-wide scale. To address this limitation, we have adapted the structural proteomics method known as limited proteolysis–mass spectrometry to identify PPIs by probing protein structural alterations within cellular extracts upon treatment with a protein of interest. Here, we added Rab GTPases, namely Rab5A and Rab2A in their GTP- and GDP-bound forms to a HEK293T cellular extract and probed their structure-specific interactomes. We identified already know interactors of these proteins bassed on previously published data as well as a number of candidate interactor proteins.
HostingRepositoryPRIDE
AnnounceDate2024-02-10
AnnouncementXMLSubmission_2024-02-10_15:33:32.898.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAleš Holfeld
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-01-24 12:59:14ID requested
12024-02-10 15:33:33announced
22024-10-22 06:29:01announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: LC-MSMS, Interactome, Mass spectrometry,Limited proteolysis, Structural proteomics, Protein-protein interactions, Proteomics, Rab GTPases, Structure-specific interactions
Contact List
Prof. Paola Picotti
contact affiliationInstitute of Molecular Systems Biology, ETH Zurich
contact emailpicotti@imsb.biol.ethz.ch
lab head
Aleš Holfeld
contact affiliationInstitute of Molecular Systems Biology, Department of Biology, ETH Zurich, Zurich, Switzerland
contact emailholfeld@imsb.biol.ethz.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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