PXD048848-2

PXD048848 is an original dataset announced via ProteomeXchange.

Title	Remodelling of cytoskeleton and plasma membrane proteins contributes to drought sensitivity of Arabidopsis rhd2 mutant
Description	NADPH oxidases are enzymes localised in the plasma membrane and emitting superoxide to the extracellular space. By production of superoxide as one type of reactive oxygen species (ROS), they exert pleiotropic functions in plant development and various stress responses. Arabidopsis thaliana RESPIRATORY BURST OXIDASE HOMOLOG PROTEIN C/ROOT HAIR DEFECTIVE 2 (AtRBOHC/RHD2) is an NADPH oxidase with preferential gene expression in roots. Polar localisation and ROS production by this enzyme are essential for root hair elongation. However, the proteome-wide and physiological consequences of RBOHC/RHD2 mutations are unknown. To find out potential new functions of AtRBOHC/RHD2, we employed a differential proteomic analysis of Arabidopsis rhd2-1 mutant, carrying a loss-of-function mutation in RBOHC/RHD2. Proteomic analyses that were validated with independent biochemical, phenotypical and advanced microscopy methods, showed quantitative deregulation of proteins involved in abiotic and biotic stress response, metabolism, vesicular transport and cell wall modification. Considerable differences in the differential proteomes between roots and above-ground parts were found in the mutant. The altered abundance of aquaporins and homeostasis of transmembrane pumps and transporters most likely determine the higher sensitivity of Arabidopsis rhd2-1 mutant to drought.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_07:00:30.025.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tibor Pechan
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-24 10:49:39	ID requested
1	2024-10-02 11:40:42	announced
⏵ 2	2024-10-22 07:00:30	announced	2024-10-22: Updated project metadata.

10.1016/J.PLAPHY.2024.109137;

submitter keyword: LC-MSMS, cytoskeleton,Arabidopsis, plasma, stress, membrane, drought, abiotic

Tibor Pechan
contact affiliation	Mississippi State University
contact email	pechan@ra.msstate.edu
lab head
Tibor Pechan
contact affiliation	IGBB, Mississippi State University
contact email	pechan@ra.msstate.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048848
     1. Label: PRIDE project
     2. Name: Remodelling of cytoskeleton and plasma membrane proteins contributes to drought sensitivity of Arabidopsis rhd2 mutant