PXD048829-1

PXD048829 is an original dataset announced via ProteomeXchange.

Title	Fibroblasts’ secretome from calcified and non-calcified dermis in Pseudoxanthoma elasticum differently contributes to elastin calcification.
Description	Pseudoxanthoma elasticum (PXE) is characterized by ectopic calcification, however, despite the widely spread effect of pro/anti-calcifying systemic factors associated with the genetic metabolic condition, it not known why elastic fibers (EF) in the same patient are mainly fragmented or highly mineralized in clinically unaffected (CUS) and affected (CAS) skin, respectively. Cellular morphology and secretome were investigated in vitro in CUS and CAS fibroblasts. Data reveal that in CAS fibroblasts focal adhesions and stress fibers are differently distributed and organized thus affecting cell-matrix interactions (i.e., collagen gel retraction). These changes are known to influence the extracellular matrix (ECM) and the signals provided to cells. Differentially expressed secreted proteins (e.g., altered balance of MMPs/TIMP, of pro-/anti-calcifying proteoglycans, of elastic-fibers associated glycoproteins) may alter the stability of EF and the ECM milieu, creating local microenvironments guiding the level of matrix remodeling at an extent that may lead to degradation (in CUS) or to degradation and calcification (in CAS) of the elastic component, further highlighting the active role of fibroblasts and of ECM in pathologic mineral deposition.
HostingRepository	PRIDE
AnnounceDate	2024-04-18
AnnouncementXML	Submission_2024-04-18_02:47:04.293.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francesco Demetrio Lofaro
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-24 01:49:29	ID requested
⏵ 1	2024-04-18 02:47:04	announced
2	2024-10-22 06:36:33	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: fibroblasts

elastin

mineralization

proteomics

PXE

Federica Boraldi
contact affiliation	Life Sciences, University of Modena and Reggio Emilia
contact email	federica.boraldi@unimore.it
lab head
Francesco Demetrio Lofaro
contact affiliation	University of Modena and Reggio Emilia
contact email	francescodemetrio.lofaro@unimore.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD048829

PRIDE project URI

• PRIDE
  1. PXD048829
     1. Label: PRIDE project
     2. Name: Fibroblasts’ secretome from calcified and non-calcified dermis in Pseudoxanthoma elasticum differently contributes to elastin calcification.