PXD048811 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Electron Capture vs. Transfer Dissociation for Site Determination of Tryptic Peptide Tyrosine Sulfation: Direct Detection of Fibrinogen Sulfation Sites and Identification of Novel Isobaric Interferences |
Description | Tyrosine sulfation is an understudied posttranslational modification (PTM) of crucial biological significance. Conventional liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods are unable to directly identify sulfation sites due to the high lability of the sulfate PTM. In this study, we were able to directly detect and localize tyrosine sulfation sites in a proteomics workflow. To meet that objective, we combined bottom-up nanoflow LC-beam-type CID (nanoLC-HCD) with conventional “MSFragger-Labile” analysis, nanoLC-electron transfer with supplemental HCD (EThcD) MS/MS, and LC-electron capture dissociation (ECD) MS/MS for the characterization of bovine fibrinogen sulfopeptide candidates from both purified protein and from plasma. |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-21 |
AnnouncementXML | Submission_2024-06-20_19:19:09.559.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD048811 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Menatallah Youssef |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; |
ModificationList | O4'-sulfo-L-tyrosine |
Instrument | Orbitrap Fusion Lumos; Bruker Daltonics solarix series |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-01-23 11:03:00 | ID requested | |
⏵ 1 | 2024-06-20 19:19:09 | announced | |
Publication List
Keyword List
submitter keyword: post-translational modifications, mass spectrometry, direct determination,Tyrosine sulfation, proteomics. |
Contact List
Kristina Hakansson |
contact affiliation | Department of Chemistry, University of Michigan, Ann Arbor, MI, United States |
contact email | kicki@umich.edu |
lab head | |
Menatallah Youssef |
contact affiliation | Department of Chemistry, University of Michigan |
contact email | menaty@umich.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048811
- Label: PRIDE project
- Name: Electron Capture vs. Transfer Dissociation for Site Determination of Tryptic Peptide Tyrosine Sulfation: Direct Detection of Fibrinogen Sulfation Sites and Identification of Novel Isobaric Interferences