PXD048800 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Pmk1 kinase-dependent phosphosite mapping on Slp1 |
Description | In late mitosis, the timely polyubiquitylation and subsequent degradation of securin and cyclin B by anaphase-promoting complex/cyclosome (APC/C) play a critical role for anaphase onset and chromosome segregation. Binding of Cdc20, the co-activator of the APC/C, is critical for activation of APC/C at anaphase entry. We found that one MAPK, Pmk1, is involved in phosphorylation of Slp1Cdc20 (the Cdc20 homologue in the fission yeast Schizosaccharomyces pombe), which may promote its ubiquitylation and subsequent degradation, and thus impede APC/C activation and anaphase entry. To gain a full picture of Pmk1-dependent phosphosite map of Slp1Cdc20, we purified the APC/C-associated Slp1Cdc20 by immunoprecipitation of one APC/C subunit Apc15 in metaphase-arrested nda3-km311 cells. Apc15-GFP was purified from wild type, pek1DD [i.e. pek1(S234D,T238D)] and pmk1∆ cells respectively. Samples were separated by SDS-PAGE, and Coomassie blue-stained gel slices were excised and submitted for mass spectrometry analyses. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_07:01:56.526.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Quan-wen Jin |
SpeciesList | scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896; |
ModificationList | phosphorylated residue |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-01-23 05:05:28 | ID requested | |
1 | 2024-10-15 03:37:43 | announced | |
⏵ 2 | 2024-10-22 07:01:56 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Slp1,Pmk1, phosphorylation, fission yeast (Schizosaccharomyces pombe) |
Contact List
Quan-wen Jin |
contact affiliation | State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, Fujian, China |
contact email | jinquanwen@xmu.edu.cn |
lab head | |
Quan-wen Jin |
contact affiliation | School of Life Sciences, Xiamen University |
contact email | jinquanwen@xmu.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048800
- Label: PRIDE project
- Name: Pmk1 kinase-dependent phosphosite mapping on Slp1