PXD048800-2

PXD048800 is an original dataset announced via ProteomeXchange.

Title	Pmk1 kinase-dependent phosphosite mapping on Slp1
Description	In late mitosis, the timely polyubiquitylation and subsequent degradation of securin and cyclin B by anaphase-promoting complex/cyclosome (APC/C) play a critical role for anaphase onset and chromosome segregation. Binding of Cdc20, the co-activator of the APC/C, is critical for activation of APC/C at anaphase entry. We found that one MAPK, Pmk1, is involved in phosphorylation of Slp1Cdc20 (the Cdc20 homologue in the fission yeast Schizosaccharomyces pombe), which may promote its ubiquitylation and subsequent degradation, and thus impede APC/C activation and anaphase entry. To gain a full picture of Pmk1-dependent phosphosite map of Slp1Cdc20, we purified the APC/C-associated Slp1Cdc20 by immunoprecipitation of one APC/C subunit Apc15 in metaphase-arrested nda3-km311 cells. Apc15-GFP was purified from wild type, pek1DD [i.e. pek1(S234D,T238D)] and pmk1∆ cells respectively. Samples were separated by SDS-PAGE, and Coomassie blue-stained gel slices were excised and submitted for mass spectrometry analyses.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_07:01:56.526.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Quan-wen Jin
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896;
ModificationList	phosphorylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-23 05:05:28	ID requested
1	2024-10-15 03:37:43	announced
⏵ 2	2024-10-22 07:01:56	announced	2024-10-22: Updated project metadata.

10.7554/ELIFE.97896;

submitter keyword: Slp1,Pmk1, phosphorylation, fission yeast (Schizosaccharomyces pombe)

Quan-wen Jin
contact affiliation	State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, Fujian, China
contact email	jinquanwen@xmu.edu.cn
lab head
Quan-wen Jin
contact affiliation	School of Life Sciences, Xiamen University
contact email	jinquanwen@xmu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048800
     1. Label: PRIDE project
     2. Name: Pmk1 kinase-dependent phosphosite mapping on Slp1